Chitosan nerve conduits seeded with autologous bone marrow mononuclear cells for 30 mm goat peroneal nerve defect.
Chitosan nerve conduits seeded with autologous bone marrow mononuclear cells for 30 mm goat peroneal nerve defect.
复制标题
壳聚糖神经导管植入自体骨髓单核细胞治疗30mm山羊腓神经缺损
DOI：
10.1038/srep44002
复制
发表时间：
2017-03-13
影响因子：
4.6
通讯作者：
Ao Q
中科院分区：
文献类型：
--
作者：
Muheremu A;Chen L;Wang X;Wei Y;Gong K;Ao Q
本研究为探讨壳聚糖神经导管与自体骨髓单核细胞（BM-MNCs）复合能否用于山羊30 mm长的腓神经缺损的桥接，将15只动物分为BM-MNC组（n = 5）、载体组（n = 5）和自体神经移植物组（n = 5）。术后12个月，采用行为学观察、磁共振成像检查、组织形态学和电生理分析对动物进行评价。结果显示：BM-MNC组和自体神经移植组动物功能恢复良好；磁共振成像和组织形态学分析表明，神经缺损由髓鞘神经轴突桥接。两组间有髓轴突密度、轴突直径、髓鞘厚度及腓神经动作电位均无显著差异。载药组动物功能恢复不明显。结果表明，自体骨髓单核细胞植入壳聚糖神经导管在长周围神经缺损的桥接方面具有较强的潜力，可用于临床试验。
In the current research, to find if the combination of chitosan nerve conduits seeded with autologous bone marrow mononuclear cells (BM-MNCs) can be used to bridge 30 mm long peroneal nerve defects in goats, 15 animals were separated into BM-MNC group (n = 5), vehicle group (n = 5), and autologous nerve graft group (n = 5). 12 months after the surgery, animals were evaluated by behavioral observation, magnetic resonance imaging tests, histomorphological and electrophysiological analysis. Results revealed that animals in BM-MNC group and autologous nerve graft group achieved fine functional recovery; magnetic resonance imaging tests and histomorphometry analysis showed that the nerve defect was bridged by myelinated nerve axons in those animals. No significant difference was found between the two groups concerning myelinated axon density, axon diameter, myelin sheath thickness and peroneal nerve action potential. Animals in vehicle group failed to achieve significant functional recovery. The results indicated that chitosan nerve conduits seeded with autologous bone marrow mononuclear cells have strong potential in bridging long peripheral nerve defects and could be applied in future clinical trials.