Simultaneous Determination of Four Aflatoxins in Dark Tea by Multifunctional Purification Column and Immunoaffinity Column Coupled to Liquid Chromatography Tandem Mass Spectrometry
Simultaneous Determination of Four Aflatoxins in Dark Tea by Multifunctional Purification Column and Immunoaffinity Column Coupled to Liquid Chromatography Tandem Mass Spectrometry
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多功能纯化柱和免疫亲和柱联用液相色谱串联质谱法同时测定黑茶中4种黄曲霉毒素
DOI：
10.1021/acs.jafc.9b04933
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发表时间：
2019-10-16
影响因子：
6.1
通讯作者：
Zhou, Yu
中科院分区：
文献类型：
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作者：
Ye, Ziling;Cui, Pu;Zhou, Yu
干茶基质含有丰富的咖啡因和多酚，这与食物基质（如蛋白质、脂质和碳水化合物）不同，只有少数研究尝试用茶样品测定黄曲霉毒素。本研究建立了一种特异、准确、灵敏的方法，用于同时测定黑茶中黄曲霉毒素B1、B2、G1和G2。用乙腈/水法提取黄曲霉毒素，经加压过滤，用多功能纯化柱（MFC）和免疫亲和柱（IAC）串联纯化。采用液相色谱-串联质谱法对纯化后的提取物进行分析。MFC-IAC清洗方法可有效降低基体干扰。加标浓度为5 ~ 60 μg/kg时，加标回收率为77.5 ~ 93%，相对标准偏差为0.998。检测限为0.024 ~ 0.21 μg/kg，定量限为0.08 ~ 0.74 μg/kg。日内、日间精密度范围为74% ~ 87%，日内、日间精密度范围为0.4% ~ 3.1%。方法验证后，对所采集的100种黑茶进行了黄曲霉毒素的检测，并与其他方法进行了比较。
Dry tea matrix contains an abundance of caffeine and polyphenols which are different from the food matrix (e.g., protein, lipid, and carbohydrates), and only a few studies have tried aflatoxins determination with tea samples. Here, a specific, accurate, and sensitive method was developed and validated for the simultaneous determination of aflatoxin B1, B2, G1, and G2 in dark teas. Aflatoxins were extracted by acetonitrile/water, press-filtered, and cleaned by multifunctional purification column (MFC) and immunoaffinity column (IAC) in tandem. The cleaned extract was analyzed by liquid chromatography tandem mass spectrometry. The matrix interference was effectively reduced by MFC-IAC cleaning method. Recoveries at the spiking concentrations of 5-60 μg/kg ranged from 77.5 to 93%, with relative standard deviations 0.998. The limits of detection were 0.024-0.21 μg/kg and the limits of quantification were 0.08-0.74 μg/kg. The intra- and interday accuracy ranged from 74 to 87%, and the intra- and interday precisions ranged from 0.4 to 3.1%. After the method validation, the aflatoxins contaminations in 100 collected dark teas were detected, and the results were compared with those of other methods.