Sfrp5 interacts with Slurp1 to regulate the accumulation of triglycerides in hepatocyte steatosis model
Sfrp5 interacts with Slurp1 to regulate the accumulation of triglycerides in hepatocyte steatosis model
复制标题
Sfrp5与Slurp1相互作用调节肝细胞脂肪变性模型中甘油三酯的积累
DOI：
10.1016/j.bbrc.2019.03.035
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发表时间：
2019-04-30
影响因子：
3.1
通讯作者：
Li, Ling
中科院分区：
文献类型：
--
作者：
Zhao, Anjiang;Jiang, Feiyu;Li, Ling
分泌型卷曲相关蛋白5（Sfrp 5）是脂肪细胞分泌的一种抗炎脂肪细胞因子，在能量代谢中起重要作用。研究表明，Sfrp 5在调节脂质代谢方面发挥着有益的作用，但其具体机制还有待进一步研究。在这项研究中，我们发现饮食诱导的肥胖受试者中Sfrp 5的水平低于正常饮食的C57 BL/6 J小鼠。为了进一步研究Sfrp 5相关蛋白在HepG 2细胞中的表达，进行了免疫沉淀法和银染法。通过质谱分析，分泌的淋巴细胞抗原-6/尿激酶型纤溶酶原激活物受体相关肽（Slurp-1）被发现与Sfrp 5相互作用。通过正、反向免疫沉淀试验进一步验证。在本研究中，我们发现Slurp 1的单独过表达促进了棕榈酸诱导的HepG 2细胞中Sfrp 5的表达。此外，我们的实验证据表明，在棕榈酸诱导的模型细胞中Sfrp 5表达抑制的情况下，Slurp 1在降低TG水平中的作用大大降低。我们的研究进一步发现，Slurp 1通过与Sfrp 5相互作用来调节甘油三酯的合成途径，从而减轻棕榈酸诱导的模型细胞中甘油三酯的积累。综上所述，我们是第一个发现Sfrp 5和Slurp 1之间相互作用的人，我们发现Slurp 1可能通过Sfrp 5调节TG的积累。(C)2019爱思唯尔公司All rights reserved.
Secreted frizzled-related protein 5 (Sfrp5), an anti-inflammatory adipocytokine secreted by adipocytes, plays an important role in energy metabolism. Studies have shown that Sfrp5 plays a salutary role in the regulation of lipid metabolism, but its specific mechanism needs further study. In this study, we showed a lower level of Sfrp5 in subjects with diet-induced obesity than in normal-diet C57BL/6J mice. To further investigate Sfrp5-associated proteins in HepG2 cells, the immunoprecipitation assay and silver staining assay were performed. By mass spectrometry analysis, secreted lymphocyte antigen-6/urokinase-type plasminogen activator receptor-related peptide (Slurp-1) was found to interact with Sfrp5. Further verification was obtained through the positive and reverse immunoprecipitation assay. In this study, we found that the sole over-expression of Slurp1 promoted the expression of Sfrp5 in palmitate-induced HepG2 cells. In addition, our experimental evidence shows that the role of Slurp1 in decreasing TG level was greatly reduced in the case of suppression of expression of Sfrp5 in palmitate-induced model cells. Our study further found that Slurp1 regulates the synthesis pathway of triglyceride by interacting with Sfrp5 to alleviate triglyceride accumulation in palmitate-induced model cells. In summary, we are the first to discover the interaction between Sfrp5 and Slurp1, and we found that Slurp1 may regulate the accumulation of TG through Sfrp5. (C) 2019 Elsevier Inc. All rights reserved.