Dose-dependent effect of Lactobacillus rhamnosus on quantitative reduction of faecal rotavirus shedding in children
Dose-dependent effect of Lactobacillus rhamnosus on quantitative reduction of faecal rotavirus shedding in children
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DOI：
10.1093/tropej/fmp001
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发表时间：
2009-10-01
影响因子：
2
通讯作者：
Fang, Hsu-Wei
中科院分区：
文献类型：
--
作者：
Fang, Shiuh-Bin;Lee, Hung-Chang;Fang, Hsu-Wei
益生菌对儿童急性感染性腹泻的有益效果主要见于水样腹泻和病毒性胃肠炎。鼠李糖乳杆菌是研究最广泛的益生菌之一，能有效缩短儿童急性腹泻的病程。然而，乳杆菌对人类粪便轮状病毒排泄量的剂量依赖效应仍鲜为人知。因此，对23例急性轮状病毒胃肠炎患儿进行了一项开放标签随机试验，随机分配患者接受三种方案中的一种，为期3天：对照组6例，每日给予0 CFU的鼠李糖乳杆菌35(LCr35)；低剂量组9例，每日2×10(8)CFU；高剂量组8例，每日6×10(8)CFU。在3天疗程前和3天后采集粪便样本，用酶联免疫吸附试验测定轮状病毒浓度。无论是对照组(119.2×10(5)个颗粒/毫升vs.23.7x10(5)个颗粒/毫升，p=0.075)或低剂量组(36.1x10(5)个颗粒/毫升vs.73.5x10(5)个颗粒/毫升，p=0.859)，粪便轮状病毒浓度都没有统计学意义的变化。然而，高剂量组的粪便轮状病毒浓度显著降低(64.2x10(5)颗粒/毫升vs.9.0x10(5)颗粒/毫升，p=0.012)。无一例外，大剂量Lcr35组所有8名患者的粪便轮状病毒浓度在3天后与Lcr35治疗前相比下降了86%。总之，这是首次提供鼠李糖乳杆菌(最小有效剂量为6×10(8)CFU，持续3天)对儿科患者粪便轮状病毒脱落的剂量依赖效应的定量证据。
Beneficial effects of probiotics in acute infectious diarrhoea in children are mainly seen in watery diarrhoea and viral gastroenteritis. Lactobacillus rhamnosus, one the most extensively studied probiotic strains, is effective in shortening courses of acute diarrhoea in children. However, the dose-dependent effect of Lactobacillus upon quantification of faecal rotavirus shedding in humans remains little known. Thus, an open-label randomized trial in 23 children with acute rotaviral gastroenteritis was undertaken by randomly allocating patients to receive one of the three regimens for 3 days: daily Lactobacillus rhamnosus 35 (Lcr35) with 0 CFU/day to six patients in the control group, 2 x 10(8) CFU/day to nine patients in the low-dose group, and 6 x 10(8) CFU/day to eight patients in the high-dose group. Faecal samples were collected before and after the 3-day regimen for measurements of rotavirus concentrations by ELISA. There was no statistically significant change in faecal rotavirus concentrations in either the control group (119.2 x 10(5) particles/ml vs. 23.7 x 10(5) particles/ml, p=0.075) or the low-dose group (36.1 x 10(5) particles/ml vs. 73.5 x 10(5) particles/ml, p=0.859). However, the high-dose group had a significant reduction of faecal rotavirus concentration (64.2 x 10(5) particles/ml vs. 9.0 x 10(5) particles/ml, p=0.012). Without any exception, the faecal rotavirus concentrations of all eight patients in the high-dose Lcr35 group declined by 86% after 3 days when compared with those before Lcr35 administration. In conclusion, this is the first report to provide quantitative evidence of the dose-dependent effect of Lactobacillus rhamnosus, a minimal effective dose of 6 x 10(8) CFU for 3 days, upon the faecal rotavirus shedding in paediatric patients.