EUROPIUM AS A LABEL IN TIME-RESOLVED IMMUNOFLUOROMETRIC ASSAYS
EUROPIUM AS A LABEL IN TIME-RESOLVED IMMUNOFLUOROMETRIC ASSAYS
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DOI：
10.1016/0003-2697(84)90095-2
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发表时间：
1984-01-01
影响因子：
2.9
通讯作者：
LOVGREN, T
中科院分区：
文献类型：
--
作者：
HEMMILA, I;DAKUBU, S;LOVGREN, T
非同位素免疫分析的基础上开发的灵敏检测Eu（III）在水溶液中使用时间分辨荧光法。Eu标记物与抗体和EDTA衍生物（重氮苯基-EDTA-Eu或异硫氰酸基苯基-EDTA-Eu）结合。在免疫测定完成后，Eu优选在低pH下与抗体解离，并通过在含有Triton X-100、β-葡聚糖、葡聚糖和葡聚糖的胶束溶液中的时间分辨荧光进行测量。二酮和刘易斯碱。去污剂溶解溶液中的螯合化合物，并从荧光配体-Eu络合物中排除水。Eu浓度低至5 μ m。使用1秒计数时间测量10-14 M。以兔IgG为模型体系的免疫测定灵敏度为25 pg/ml（6 pg/次）。
A nonisotopic immunoassay was developed based on a sensitive detection of Eu(III) in water solution using time-resolved fluorometry. The Eu label is bound to the antibody and EDTA derivatives, either diazophenyl-EDTA-Eu or isothiocyanatophenyl-EDTA-Eu. After the immunometric assay has been completed the Eu is preferably dissociated from the antibody at low pH and measured by time-resolved fluorescence in a micellar solution containing Triton X-100, .beta.-diketone and a Lewis base. The detergent solubilizes the chelating compounds in the solution and excludes water from the fluorescent ligand-Eu complex. Eu concentrations as low as 5 .cntdot. 10-14 M were measured using a 1-s counting time. The sensitivity of the immunoassay of rabbit IgG used as a model system was 25 pg/ml (6 pg/assay).