Effect of dietary fat on fat absorption and concomitant plasma and tissue fat composition in a rat model of short bowel syndrome
Effect of dietary fat on fat absorption and concomitant plasma and tissue fat composition in a rat model of short bowel syndrome
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DOI：
10.1007/s00383-004-1143-5
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发表时间：
2004-03-01
影响因子：
1.8
通讯作者：
Harmon, CM
中科院分区：
文献类型：
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作者：
Sukhotnik, I;Mor-Vaknin, N;Harmon, CM
本研究的目的是探讨在短肠综合征（SBS）大鼠模型中，膳食脂肪对脂肪吸收和组织及血浆脂质组成变化的时间过程的影响。雄性Sprague-Dawley大鼠进行肠横断再吻合术（Sham大鼠）或75%小肠切除术（SBS大鼠）。将动物随机分配到三个组中的一个：喂食正常食物的Sham大鼠（Sham-NC）、喂食正常食物的SBS大鼠（SBS-NC）或喂食高脂肪饮食的SBS大鼠（SBS-HFD）。在第3天或第14天处死大鼠。每周两次测量体重、摄食量、食物清除率（干粪便量）和脂肪清除率（总粪便脂肪）。脂肪和能量的摄入量根据摄入的食物量计算。食物和脂肪吸收率计算为摄入量减去清除率，并表示为摄入量的百分比。测定血清胆固醇、甘油三酯和白蛋白。测定肝脏、附睾脂肪组织和小肠的总脂质组成。通过Student检验进行统计学分析，p值
The aim of this study was to investigate the effect of dietary fat on the time course of changes in fat absorption and tissue and plasma lipid composition in a rat model of short bowel syndrome (SBS). Male Sprague-Dawley rats underwent either a bowel transection with re-anastomosis (Sham rats) or 75% small bowel resection (SBS rats). Animals were randomly assigned to one of three groups: Sham rats fed normal chow (Sham-NC), SBS rats fed normal chow (SBS-NC), or SBS rats fed a high-fat diet (SBS-HFD). Rats were sacrificed on day 3 or 14. Body weight, food intake, food clearance (dry fecal mass), and fat clearance (total fecal fat) were measured twice a week. Fat and energy intakes were calculated according to the amount of ingested food. Food and fat absorbability were calculated as intake minus clearance and were expressed as percent of intake. Serum cholesterol, triglyceride, and albumin were measured. Total lipid composition of the liver, epididymal adipose tissue, and the small intestine was determined. Statistical analysis was performed by a Student's test, with p values