Evaluation of the anti-cancer activity of the triterpenoidal saponin fraction isolated from the traditional Chinese medicine Conyza blinii H. Lev
Evaluation of the anti-cancer activity of the triterpenoidal saponin fraction isolated from the traditional Chinese medicine Conyza blinii H. Lev
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DOI：
10.1039/c6ra26361e
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发表时间：
2017-01-01
期刊：
影响因子：
3.9
通讯作者：
Diao, Aipo
中科院分区：
文献类型：
--
作者：
Ma, Long;Liu, Haiyan;Diao, Aipo
小飞蓬利未记在中国传统上被用作治疗炎症的药用植物。我们在这里表明，这种植物的活性部分，Conyza blinii皂苷（CBS），也具有有效的抗癌活性，在体外和体内。基于细胞的实验表明，CBS以多种方式抑制癌细胞的生长。CBS以剂量依赖性方式诱导细胞凋亡。线粒体膜电位（Delta Psi（m））可被CBS破坏，这表明线粒体凋亡途径的激活。这一点通过扫描电子显微镜观察到的凋亡小体数量增加得到证实。CBS能够抑制癌细胞的迁移和侵袭。流式细胞术实验表明CBS导致癌细胞S期阻滞。这些作用共同导致对HeLa细胞的抑制活性。进行了体内动物实验，结果表明，15 mg/kg CBS是一个可耐受的剂量，在10天的给药方案中，肿瘤重量减少了70%。
Conyza blinii H. Lev. is traditionally used in China as a medicinal plant to treat inflammation. We show here that the active fraction of this plant, Conyza blinii saponin (CBS), also has potent anti-cancer activity both in vitro and in vivo. Cell-based experiments showed that CBS suppresses the growth of cancer cells in a number of ways. CBS induces apoptosis in a dose-dependent manner. The mitochondrial membrane potential (Delta Psi(m)) can be disrupted by CBS, which indicates activation of the mitochondrial apoptosis pathway. This was confirmed by an increase in the number of apoptotic bodies seen with scanning electron microscopy. CBS is capable of inhibiting cancer cell migration and invasion. Flow cytometry experiments demonstrated that CBS causes S phase arrest in cancer cells. These effects collectively led to an inhibitory activity against HeLa cells. In vivo animal experiments were carried and showed that 15 mg kg(-1) CBS is a tolerable dose and reduced the tumour weight by 70% in a 10 day administration regimen.