Synthesis of an immunosuppressant SQAG9 and determination of the binding peptide by T7 phage display.
Synthesis of an immunosuppressant SQAG9 and determination of the binding peptide by T7 phage display.
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DOI：
10.1016/j.bmcl.2004.05.085
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发表时间：
2004-08
影响因子：
2.7
通讯作者：
T. Yamazaki;Satoko Aoki;K. Ohta;Shinji Hyuma;K. Sakaguchi;F. Sugawara
中科院分区：
文献类型：
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作者：
T. Yamazaki;Satoko Aoki;K. Ohta;Shinji Hyuma;K. Sakaguchi;F. Sugawara
合成了一类新的免疫抑制磺基喹诺酮基甘油SQAG9及其生物素化衍生物。由黑腹果蝇随机cDNA片段组成的T7噬菌体文库显示了一个可能的14个氨基酸的结合肽。在表面等离子体共振实验中，传感器芯片上固定化的合成肽对SQAG9的解离常数为Kd=1.5×10−6。
SQAG9, a new class of immunosuppressive sulfoquinovosylacylglycerol, and its biotinylated derivatives have been synthesized. A T7 Phage library, composed of random cDNA fragments from Drosophila melanogaster, displayed a possible binding peptide of 14 amino acids. The immobilized synthetic peptide on a sensor chip showed a dissociation constant of KD=1.5×10−6against SQAG9 in a surface plasmon resonance experiment.