The development of cryo-EM into a mainstream structural biology technique.
The development of cryo-EM into a mainstream structural biology technique.
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DOI：
10.1038/nmeth.3694
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发表时间：
2016-01
期刊：
影响因子：
48
通讯作者：
Nogales E
中科院分区：
文献类型：
--
作者：
Nogales E
单粒子低温电子显微镜(Cryo-EM)是近二十年来出现的一种能够研究具有挑战性的体系的技术，否则这些体系就无法进行结构表征。最近的技术进步在适用性、吞吐量和可实现的分辨率方面取得了巨大的飞跃，使这项技术获得了全世界的关注。在这里，我将讨论低温EM领域发展过程中的一些主要历史里程碑，最终导致其目前的成功。
Single-particle cryo-electron microscopy (cryo-EM) has emerged over the last two decades as a technique capable of studying challenging systems that otherwise defy structural characterization. Recent technical advances have resulted in a ‘quantum leap’ in applicability, throughput and achievable resolution that has gained this technique worldwide attention. Here I discuss some of the major historical landmarks in the development of the cryo-EM field, ultimately leading to its present success.