Differential expression of Ia molecules by human monocytes.
Differential expression of Ia molecules by human monocytes.
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人类单核细胞 Ia 分子的差异表达。
DOI：
10.1172/jci111503
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Stobo,JD
中科院分区：
文献类型：
--
作者：
Gonwa,TA;Stobo,JD
人类免疫应答基因可分为三个不同的基因座，每个基因座编码三个不同的Ia分子家族：HLA-SB、HLA-DC和HLA-DR。使用单克隆抗体，我们研究了粘附的人外周血单核细胞的HLA-DR和HLA-DC分子的展示。这些研究的结果表明，虽然所有人外周血单核细胞显示容易检测的HLA-DR分子，只有50%显示容易检测的HLA-DC分子。将外周血单核细胞分离成HLA-DC+和HLA-DC-细胞表明，每个群体显示相等密度的HLA-DR分子。因此，基于它们对这两种Ia分子的展示的差异，粘附的外周血单核细胞可分为两大群体：HLA-DR+、HLA-DC+和HLA-DR+、HLA-DC-。尽管这些Ia抗原的不协调展示，但HLA-DR和HLA-DC两者的表达可由共同信号γ干扰素（IFN-γ）调节。单核细胞在自体血清中孵育96小时导致HLA-DR和HLA-DC的表达显著降低。向培养物中加入重组IFN-γ导致HLA-DR和HLA-DC的再表达达到与新鲜单核细胞中所见相当的水平。此外，尽管IFN-γ不调节所有单核细胞表面标志物，但可以证明其以与Ia抗原所观察到的类似的方式调节一种标志物MAC 120的表达。这些研究表明，在人外周血单核细胞中，Ia分子HLA-DC的分布与HLA-DR的分布并不协调，尽管两者都响应于相同的调节信号。
Human immune response genes can be divided into three distinct loci, each of which codes for three distinct families of Ia molecules: HLA-SB, HLA-DC, and HLA-DR. The tissue distribution and function of only one of these Ia molecules, HLA-DR, has been thoroughly studied. Using monoclonal antibodies, we examined the display of HLA-DR and HLA-DC molecules by adherent, human peripheral blood monocytes. The results of these studies demonstrate that although all human peripheral blood monocytes display easily detectable HLA-DR molecules, only 50% display easily detectable HLA-DC molecules. Separation of peripheral blood monocytes into HLA-DC+ and HLA-DC- cells demonstrates that each population displays an equivalent density of HLA-DR molecules. Therefore, on the basis of differences in their display of these two Ia molecules, adherent peripheral blood monocytes can be divided into two broad populations: HLA-DR+, HLA-DC+, and HLA-DR+, HLA-DC-. Despite the dis-coordinate display of these Ia antigens, the expression of both HLA-DR and HLA-DC can be regulated by a common signal, gamma interferon (IFN-gamma). Incubation of monocytes for 96 h in autologous serum leads to a marked decrease in the expression of both HLA-DR and HLA-DC. Addition of recombinant IFN-gamma to the cultures leads to reexpression of both HLA-DR and HLA-DC to levels comparable to those seen in fresh monocytes. In addition, although IFN-gamma does not modulate all monocyte surface markers, it can be demonstrated to modulate expression of one marker, MAC 120, in a manner similar to that observed for Ia antigens. These studies demonstrate that among human peripheral blood monocytes, the distribution of the Ia molecule, HLA-DC, is not coordinate with that of HLA-DR, although both respond to the same regulatory signal.