Recombinant feline herpesvirus type 1 expressing immunogenic proteins inducible virus neutralizing antibody against feline calicivirus in cats.
Recombinant feline herpesvirus type 1 expressing immunogenic proteins inducible virus neutralizing antibody against feline calicivirus in cats.
复制标题
重组猫疱疹病毒 1 型，表达猫体内针对猫杯状病毒的免疫原性蛋白诱导病毒中和抗体。
DOI：
10.1016/s0264-410x(96)00131-4
复制
发表时间：
1996
期刊：
影响因子：
5.5
通讯作者：
T. Mikami
中科院分区：
文献类型：
--
作者：
N. Yokoyama;K. Maeda;Y. Tohya;Y. Kawaguchi;K. Fujita;T. Mikami
本研究将编码猫杯状病毒(FCV)F4株衣壳蛋白的完整开放阅读框插入猫疱疹病毒1型(FHV-1)胸苷酶(TK)缺失突变株(C7301dlTK)的SmaI位点，构建成重组病毒C7301dlTK-Cap。间接免疫荧光法和免疫印迹分析证实C7301dlTK-Cap表达FCV抗原。为了评价重组病毒能否在自然宿主中诱导抗FCV的病毒中和抗体，将C7301dlTK-Cap(两只猫)和C7301dlTK(一只猫)分别经鼻腔和口服接种给3只猫。结果表明，接种C7301dlTK-Cap的猫血清中有抗FCV的VN抗体。该重组病毒有望成为针对FHV-1和FCV感染的新型多价重组疫苗。
In this study, an entire open reading frame encoding the capsid protein of feline calicivirus (FCV) F4 strain was inserted into the deletion locus (SmaI site) of the thymidine kinase (TK) deficient mutant (C7301dlTK) of feline herpesvirus type 1 (FHV-1) and the resulting recombinant virus was designated as C7301dlTK-Cap. Expression of the FCV antigens by C7301dlTK-Cap was confirmed by indirect immunofluorescence assay and immunoblot analysis. To assess whether the recombinant virus can induce virus neutralizing (VN) antibody against FCV in the natural host, three cats were inoculated intranasally and orally with C7301dlTK-Cap (two cats) or C7301dlTK (one cat). As a result, sera collected from cats inoculated with the C7301dlTK-Cap possessed VN antibody against FCV. This recombinant virus is expected as a new polyvalent recombinant vaccine against FHV-1 and FCV infections.