Heat Shock Protein 47 Promotes Glioma Angiogenesis
Heat Shock Protein 47 Promotes Glioma Angiogenesis
复制标题
热休克蛋白 47 促进神经胶质瘤血管生成
DOI：
10.1111/bpa.12256
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发表时间：
2016-01-01
期刊：
影响因子：
6.4
通讯作者：
Zhao, Wei Guo
中科院分区：
文献类型：
--
作者：
Wu, Zhe Bao;Cai, Lin;Zhao, Wei Guo
Heat shock protein 47 (HSP47) is a collagen-binding protein, which has been recently found to express in glioma vessels. However, the expression profile of HSP47 in glioma patients and the underlying mechanisms of HSP47 on glioma angiogenesis are not fully explored. In the current study, we found that expression of HSP47 in glioma vessels was correlated with the grades of gliomas. HSP47 knockdown by siRNAs significantly decreased cell viability in vitro and tumor volume in vivo; moreover, it reduced the microvessel density (MVD) by CD31 immunohistochemistry in vivo. HSP47 knockdown significantly inhibited tube formation, invasion and proliferation of human umbilical vein endothelial cells (HUVECs). Furthermore, conditional medium derived from HSP47 knockdown cells significantly inhibited HUVECs tube formation and migration, while it increased chemosensitivity of HUVECs cells to Avastin. Silencing of HSP47 decreased VEGF expression in glioma cells consistently, and reduced glioma vasculature. Furthermore, HSP47 promoted glioma angiogenesis through HIF1-VEGFR2 signaling. The present study demonstrates that HSP47 promotes glioma angiogenesis and highlights the importance of HSP47 as an attractive therapeutic target of GBM.
