Isolation and Characterization of the Bovine Corticotropin/β-Lipotropin Precursor Gene
Isolation and Characterization of the Bovine Corticotropin/β-Lipotropin Precursor Gene
复制标题
牛促肾上腺皮质激素/β-促脂激素前体基因的分离和表征
DOI：
10.1111/j.1432-1033.1981.tb06220.x
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发表时间：
2005
期刊：
影响因子：
5.4
通讯作者：
S. Numa
中科院分区：
文献类型：
--
作者：
Shigetada Nakanishi;Y. Teranishi;Y. Watanabe;M. Notake;M. Noda;H. Kakidani;H. Jingami;S. Numa
整个牛促肾上腺皮质激素/β-促脂素前体基因已被分离为一组重叠的基因组DNA片段，其延伸超过约17000个碱基对的长度。对克隆的DNA片段进行限制性内切酶图谱分析和对整个mRNA编码片段及其周围区域的核苷酸序列分析表明，促肾上腺皮质激素/β-促脂素前体基因长约7300个碱基对，含有两个插入序列;一个长度约为4000个碱基对，位于编码mRNA 5′-非翻译区的片段内，而另一个具有大约2200个碱基对的长度，在信号肽区域附近中断蛋白质编码序列。对促肾上腺皮质激素/β-促脂肪激素前体基因近端前200多个碱基对的序列分析揭示了一个“Hogness盒”和一个模型序列的变体以及在其他真核基因中观察到的回文结构。此外，在促肾上腺皮质激素/β-促脂素前体基因与小鼠α-珠蛋白和β-珠蛋白基因的5′-侧翼区发现了一些序列相似性，这些基因均受糖皮质激素的负调控。在促肾上腺皮质素/β-促脂素前体基因区域中，至少有四个同源重复序列分布在3000-5000个碱基对的距离处;两个这样的序列位于5′-侧翼区域，每个间插序列内有一个。牛垂体核RNA的印迹杂交分析表明，促肾上腺皮质激素/β-促脂肪激素前体基因1 S转录为初级hnRNA产物，然后剪接为成熟mRNA。
The entire bovine corticotropin/β-lipotropin precursor gene has been isolated as a set of overlapping genomic DNA fragments which extend over a length of approximately 17000 base pairs. Restriction mapping of the cloned DNA fragments and nucleotide sequence analysis of the whole mRNA-coding segments and their surrounding regions have established that the corticotropin/β-lipotropin precursor gene is approximately 7300-basepairs long and contains two intervening sequences; one with an approximate length of 4000 base pairs is located within the segment encoding the 5′-untranslated region of the mRNA, and the other with an approximate length of 2200 base pairs interrupts the protein-coding sequence near the signal peptide region. Sequence analysis of more than 200 base pairs preceding the proximal end of the corticotropin/(f-lipotropin precursor gene has revealed a ‘Hogness box’ and a variant of the model sequence as well as palindrome structures as observed in other eukarvotic genes. Furthermore, some sequence similarities in the 5′-flanking region are found between the corticotropin/β-lipotropin precursor gene and the mouse α-globin and β-globin genes, all of which are negatively regulated by glucocorticoids. At least four homologous repetitive sequences are distributed at 3000–5000-base-pair distances in the corticotropin/β-lipotropin precursor gene region; two such sequences are located in the 5′-flanking region, and one within each intervening sequence. Blot hybridization analysis of bovine pituitary nuclear RNA has indicated that the entire corticotropin/β-lipotropin precursor gene 1S transcribed into a primary hnRNA product, which is then spliced to forth the mature mRNA.