Non-coding RNA-Associated ceRNA Networks in a New Contrast-Induced Acute Kidney Injury Rat Model
Non-coding RNA-Associated ceRNA Networks in a New Contrast-Induced Acute Kidney Injury Rat Model
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新对比诱导急性肾损伤大鼠模型中的非编码 RNA 相关 ceRNA 网络
DOI：
10.1016/j.omtn.2019.05.011
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发表时间：
2019-09-06
影响因子：
8.8
通讯作者：
Duan, Shao-Bin
中科院分区：
文献类型：
--
作者：
Cheng, Wei;Li, Xu-Wei;Duan, Shao-Bin
造影剂诱导的急性肾损伤(CI-AKI)是血管内应用放射状造影剂后的严重并发症，介入治疗和血管造影术的最新进展重新引起了人们对详细机制的解释和有效治疗的兴趣。最近的研究表明，CI-AKI和microRNA(MiRNA)之间存在潜在的联系。然而，CI-AKI中潜在的非编码RNA相关竞争内源RNA(CERNA)对仍不清楚。在这项研究中，我们通过RNA深度测序，系统地探讨了新型脑梗塞大鼠模型中CircRNA或lncRNA相关的Cerna机制。结果显示，38个CircRNAs、12个lncRNAs、13个miRNAs和127个mRNAs的表达显著异常。我们对差异表达的mRNAs进行了基因本体论和京都百科全书的基因和基因组路径分析，然后构建了CI-AKI大鼠肾脏中与CircRNA或lncRNA相关的CERNA网络。随后，用实时定量聚合酶链式反应方法验证了构建的两条脑梗塞大鼠模型中的CerNA调控通路--NOVICE_CIRC_0004153/RNOMIR144-3P/GPNMB或NAGLU和LNC_000343/RNO-miR1956-5P/KCP。这项研究首次系统地剖析了CI-AKI大鼠肾脏中非编码RNA相关的CENA图谱。选择的非编码RNA相关的CENA网络为潜在的机制提供了新的见解，并可能深刻地影响CI-AKI的诊断和治疗。
Contrast-induced acute kidney injury (CI-AKI) is a severe complication of intravascular applied radial contrast media, and recent progress in interventional therapy and angiography has revived interest in explaining detailed mechanisms and developing effective treatment. Recent studies have indicated a potential link between CI-AKI and microRNA (miRNA). However, the potential non-coding RNA-associated-competing endogenous RNA (ceRNA) pairs involved in CI-AKI still remain unclear. In this study, we systematically explored the circRNA or lncRNA-associated-ceRNA mechanism in a new rat model of CI-AKI through deep RNA sequencing. The results revealed that the expression of 38 circRNAs, 12 lncRNAs, 13 miRNAs and 127 mRNAs were significantly dysregulated. We performed Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses for mRNAs with significantly different expression and then constructed comprehensive circRNA or lncRNA-associated ceRNA networks in kidney of CI-AKI rats. Thereafter, two constructed ceRNA regulatory pathways in this CI-AKI rat model-novel_circ_0004153/rnomiR-144-3p/Gpnmb or Naglu and LNC_000343/rno-miR1956-5p/KCP-were validated by real-time qPCR. This study is the first one to provide a systematic dissection of non-coding RNA-associated ceRNA profiling in kidney of CI-AKI rats. The selected non-coding RNA-associated ceRNA networks provide new insight for the underlying mechanism and may profoundly affect the diagnosis and therapy of CI-AKI.