Expression pattern of the class I homeobox genes in ovarian carcinoma
Expression pattern of the class I homeobox genes in ovarian carcinoma
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DOI：
10.3802/jgo.2010.21.1.29
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发表时间：
2010-03-01
影响因子：
3.9
通讯作者：
Na, Jung Yeol
中科院分区：
文献类型：
--
作者：
Hong, Jin Hwa;Lee, Jae Kwan;Na, Jung Yeol
目的：虽然一些零星的报道揭示了同源盒（HOX）基因与卵巢癌之间的联系，但目前还没有全面分析1类同源盒基因在卵巢癌中的表达模式，从而确定卵巢癌发生的候选基因。方法：对4株卵巢癌细胞系和4株正常卵巢组织中36个HOX基因的不同表达模式进行分析。采用逆转录聚合酶链反应（RT-PCR）和定量分析，选择卵巢癌细胞系中表达明显高于正常卵巢的特异性基因，加入7例卵巢癌组织标本进行western blot分析。最后，通过免疫组织化学和免疫细胞化学分析比较卵巢癌组织和正常卵巢中特异性HOX基因的表达模式。结果：36个基因中，11个基因在卵巢癌细胞系和正常卵巢组织中mRNA表达水平不同。11个基因中，只有HOXB4在卵巢癌细胞系中的表达水平显著高于正常卵巢（p=0.029）。western blot、免疫组织化学和免疫细胞化学分析表明，HOXB4仅在卵巢癌细胞系或癌组织标本中表达，而在正常卵巢中不表达。结论：HOXB4可能是参与卵巢癌发生的一个新的候选基因。
Objective: Although some sporadic reports reveal the link between the homeobox (HOX) genes and ovarian carcinoma, there is no comprehensive analysis of the expression pattern of the class 1 homeobox genes in ovarian carcinoma that determines the candidate genes involved in ovarian carcinogenesis.Methods: The different patterns of expression of 36 HOX genes were analyzed, including 4 ovarian cancer cell lines and 4 normal ovarian tissues. Using a reverse transcription-polymerase chain reaction (RT-PCR) and quantification analysis, the specific gene that showed a significantly higher expression in ovarian cancer cell lines than in normal ovaries was selected, and western blot analysis was performed adding 7 ovarian cancer tissue specimens. Finally, immunohistochemical and immunocytochemical analyses were performed to compare the pattern of expression of the specific HOX gene between ovarian cancer tissue and normal ovaries.Results: Among 36 genes, 11 genes had a different level of mRNA expression between the cancer cell lines and the normal ovarian tissues. Of the 11 genes, only HOXB4 had a significantly higher level of expression in ovarian cancer cell lines than in normal ovaries (p=0.029). Based on western blot, immunohistochemical, and immunocytochemical analyses, HOXB4 was expressed exclusively in the ovarian cancer cell lines or cancer tissue specimens, but not in the normal ovaries.Conclusion: We suggest HOXB4 may be a novel candidate gene involved in ovarian carcinogenesis.