Effect of Nanostructure of Mineralized Collagen Scaffolds on Their Physical Properties and Osteogenic Potential
Effect of Nanostructure of Mineralized Collagen Scaffolds on Their Physical Properties and Osteogenic Potential
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矿化胶原支架纳米结构对其物理性质和成骨潜力的影响
DOI：
10.1166/jbn.2014.1794
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发表时间：
2014-06-01
影响因子：
2.9
通讯作者：
Zhou, Yanheng
中科院分区：
文献类型：
--
作者：
Liu, Yan;Luo, Dan;Zhou, Yanheng
组织工程使纳米结构胶原蛋白支架的发展能够满足当前骨丢失再生的挑战。本研究分别采用常规结晶法和仿生自下而上结晶法制备了纤维外矿化和纤维内矿化胶原支架。采用原子力显微镜（AFM）观察矿化胶原支架的纳米形貌和纳米力学特性。采用激光扫描显微镜和场发射扫描电镜分析了矿化胶原支架表面的体外细胞反应。AFM成像显示，两种矿化胶原支架的纳米结构不同，包括胶原原纤维中磷灰石的大小、形态和位置。纳米力学测试表明，在干燥和潮湿条件下，具有骨样层次结构的纤维内矿化胶原支架与纤维外矿化胶原支架相比，杨氏模量显著增加。然而，这两种矿化胶原蛋白支架具有相似的热行为。从细胞培养实验来看，纤维内矿化胶原支架比纤维外矿化胶原支架具有更高的细胞增殖和碱性磷酸酶活性。本研究的最大意义在于，矿化胶原支架的纳米结构可以影响细胞的初始粘附、形态和进一步的成骨潜能。本研究将有助于我们制备用于骨移植和组织工程的新型生物材料。
Tissue engineering has enabled development of nanostructured collagen scaffolds to meet current challenges in regeneration of lost bone. In this study, extrafibrillarly-mineralized and intrafibrillarly-mineralized collagen scaffolds were fabricated separately by a conventional crystallization method and a biomimetic, bottom-up crystallization method. Atomic force microscopy (AFM) was employed to examine the nanotopography and nanomechanics of the mineralized collagen scaffolds. The in vitro cell responses to the surface of the mineralized collagen scaffolds were analyzed by laser scanning microscope and field emission scanning electron microscopy. AFM imaging showed that these two mineralized collagen scaffolds exhibited different nanostructure, including the size, morphology and location of the apatites in collagen fibrils. The nanomechanical testing demonstrated that the intrafibrillarly-mineralized collagen scaffold, with bone-like hierarchy, featured a significantly increased Young's modulus compared with the extrafibrillarly-mineralized collagen scaffold in both dry and wet conditions. However, these two mineralized collagen scaffolds had a similar thermal behavior. From the cell culture experiments, the intrafibrillarly-mineralized collagen scaffold showed higher cell proliferation and alkaline phosphatase activity than the extrafibrillarly-mineralized collagen scaffold. The utmost significance of this study is that the nanostructure of the mineralized collagen scaffolds can affect the initial cell adhesion, morphology and further osteogenic potential. The present study will help us to fabricate novel biomaterials for bone grafting and tissue engineering applications.