Completion of a parasexual cycle in Candida albicans by induced chromosome loss in tetraploid strains
Completion of a parasexual cycle in Candida albicans by induced chromosome loss in tetraploid strains
复制标题
DOI：
10.1093/emboj/cdg235
复制
发表时间：
2003-05-15
期刊：
影响因子：
11.4
通讯作者：
Johnson, AD
中科院分区：
文献类型：
--
作者：
Bennett, RJ;Johnson, AD
人类致病真菌白色念珠菌传统上被归类为二倍体、无性生物。然而，最近描述了产生四倍体交配产物的生物的交配能力形式。原则上，白念珠菌的生命周期可以通过有性过程，通过性交机制，或通过两者兼而有之。在这里，我们描述了白念珠菌四倍体菌株在酿酒酵母‘孢子形成前’培养基上的生长条件，在四倍体中诱导有效的、随机的染色体丢失。染色体丢失的产物通常是DNA含量为二倍体或非常接近二倍体的菌株。如果它们遗传了合适的MTL(类交配型)基因座，这些二倍体产物本身就具有交配能力。因此，可以在白色念珠菌中进行有效的无性繁殖循环，这一循环导致了这种有机体中遗传物质的重新分类。我们表明，这种由交配和染色体丢失组成的无性生殖循环可以在实验室中用于白念珠菌的简单遗传操作。
The human pathogenic fungus Candida albicans has traditionally been classified as a diploid, asexual organism. However, mating-competent forms of the organism were recently described that produced tetraploid mating products. In principle, the C.albicans life cycle could be completed via a sexual process, via a parasexual mechanism, or by both mechanisms. Here we describe conditions in which growth of a tetraploid strain of C.albicans on Saccharomyces cerevisiae 'pre-sporulation' medium induced efficient, random chromosome loss in the tetraploid. The products of chromosome loss were often strains that were diploid, or very close to diploid, in DNA content. If they inherited the appropiate MTL (mating-type like) loci, these diploid products were themselves mating competent. Thus, an efficient parasexual cycle can be performed in C.albicans, one that leads to the reassortment of genetic material in this organism. We show that this parasexual cycle-consisting of mating followed by chromosome loss-can be used in the laboratory for simple genetic manipulations in C.albicans.