Isolation and identification of a major urinary canthaxanthin metabolite in rats.
Isolation and identification of a major urinary canthaxanthin metabolite in rats.
复制标题
大鼠尿中主要角黄素代谢物的分离和鉴定。
DOI：
10.1024/0300-9831.69.4.268
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
A. Kistler
中科院分区：
文献类型：
--
作者：
J. Bausch;H. Liechti;G. Oesterhelt;A. Kistler
在大鼠中研究了给予放射性标记的非维生素原A类胡萝卜素黄质后的尿代谢模式。在相当复杂的HPLC尿液代谢模式中，发现了一个结合的部分。用葡糖酶解偶联物，用HPLC纯化代谢产物，GC-MS和NMR鉴定其为3-羟基-4-氧代-7，8-二氢-β-紫罗兰酮。通过与合成化合物的HPLC保留时间、UV/维斯-和NMR-光谱和GC-MS的比较，证实了该结构。
The urinary metabolic pattern after administration of the radiolabeled non-provitamin A carotenoid canthaxanthin was investigated in rats. In the rather complex HPLC urinary metabolic pattern a fraction was found which was conjugated. Deconjugation of the polar conjugates with glusulase, purification of the metabolite with HPLC and identification with GC-MS and NMR revealed that it was 3-hydroxy-4-oxo-7,8-dihydro-beta-ionone. This structure was confirmed by comparisons with HPLC retention times, UV/VIS- and NMR-spectroscopy and GC-MS of the synthesized compound.