PANTOTHENATE STUDIES .1. INTERFERENCE BY D-SERINE AND L-ASPARTIC ACID WITH PANTOTHENATE SYNTHESIS IN ESCHERICHIA-COLI
PANTOTHENATE STUDIES .1. INTERFERENCE BY D-SERINE AND L-ASPARTIC ACID WITH PANTOTHENATE SYNTHESIS IN ESCHERICHIA-COLI
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DOI：
10.1128/jb.60.6.733-745.1950
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发表时间：
1950-01-01
影响因子：
3.2
通讯作者：
DAVIS, BD
中科院分区：
文献类型：
--
作者：
MAAS, WK;DAVIS, BD
D-丝氨酸抑制E. coli BV干扰β-丙氨酸转化为泛酸。在增长预期中。在EPTs中，β-丙氨酸竞争性地逆转了抑制作用，而泛酸非竞争性地逆转了抑制作用。对于不生长的细胞悬浮液，β-丙氨酸竞争性地恢复了对泛酸合成酶系统的抑制。使用相同的方法，显示L-天冬氨酸通过干扰β-丙氨酸向泛酸的转化来增强D-丝氨酸抑制。使用生化突变体封锁在不同阶段的泛酸合成允许测试的某些假设的抑制分析，与他们得到的验证性结果加强了与expts得出的结论的有效性。在野生型。生化突变体结合抑制分析的抗菌作用的机制的研究中的有用性进行了讨论。
D-serine inhibits growth of the W strain of E. coli bv interfering with the conversion of beta-alanine to pantothenate. In growth expts. the inhibition was reversed competitively by beta-alanine and non-competitively by pantothenate; in expts. with non-growing cell suspensions, inhibition of the pantothenate synthesizing enzyme system was restored competitively by beta-alanine. Using the same methods L-aspartic acid was shown to enhance D-serine inhibition by interfering with the conversion of beta-alanine to pantothenate. The use of biochemical mutants blocked at various stages of pantothenate synthesis permitted testing of certain assumptions underlying inhibition analysis; confirmatory results obtained with them strengthened the validity of the conclusions reached with expts. on the wild type. The usefulness of biochemical mutants combined with inhibition analysis in the study of the mechanism of antimicrobial action is discussed.