Differential effects of aldosterone and ADH on intracellular electrolytes in the toad urinary bladder epithelium.
Differential effects of aldosterone and ADH on intracellular electrolytes in the toad urinary bladder epithelium.
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醛固酮和抗利尿激素对蟾蜍膀胱上皮细胞内电解质的不同影响。
DOI：
10.1007/bf01872842
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Dörge,A
中科院分区：
文献类型：
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作者：
Rick,R;Spancken,G;Dörge,A
采用定量电子探针分析比较醛固酮和ADH对蟾蜍膀胱上皮细胞内电解质浓度的影响。利用能量色散 X 射线微量分析对薄冻干冷冻切片进行测量。使用醛固酮后，9 次实验中有 8 次检测到细胞内 Na 浓度出现统计学上显着的增加。颗粒细胞的平均Na浓度从8.9±1.3增加至13.2±2.2mmol/kg湿重。在 ADH 同等刺激跨上皮 Na 转运后，观察到明显更大的 Na 增加。平均而言，颗粒细胞中的 Na 浓度从 12.0±2.3 增加至 31.4±9.3 mmol/kg 湿重（5 次实验）。我们从这些结果得出结论，醛固酮除了对心尖钠流入有刺激作用外，还对钠泵产生刺激作用。基于颗粒细胞 Cl 浓度显着降低，我们讨论了泵的刺激是由醛固酮诱导的碱化介导的可能性。在基底细胞中观察到类似但不太明显的浓度变化，表明这种细胞类型也参与跨上皮 Na 转运。对富含线粒体的细胞的测量没有提供一致的结果。
Quantitative electron microprobe analysis was employed to compare the effects of aldosterone and ADH on the intracellular electrolyte concentrations in the toad urinary bladder epithelium. The measurements were performed on thin freeze-dried cryosections utilizing energy dispersive x-ray microanalysis. After aldosterone, a statistically significant increase in the intracellular Na concentration was detectable in 8 out of 9 experiments. The mean Na concentration of granular cells increased from 8.9±1.3 to 13.2±2.2 mmol/kg wet wt. A significantly larger Na increase was observed after an equivalent stimulation of transepithelial Na transport by ADH. On average, the Na concentration in granular cells increased from 12.0±2.3 to 31.4±9.3 mmol/kg wet wt (5 experiments). We conclude from these results that aldosterone, in addition to its stimulatory effect on the apical Na influx, also exerts a stimulatory effect on the Na pump. Based on a significant reduction in the Cl concentration of granular cells, we discuss the possibility that the stimulation of the pump is mediated by an aldosterone-induced alkalinization.Similar though less pronounced concentration changes were observed in basal cells, suggesting that this cell type also participates in transepithelial Na transport. Measurements in mitochondria-rich cells provided no consistent results.