Complete sequence of a cDNA encoding an active rat choline acetyltransferase: A tool to investigate the plasticity of cholinergic phenotype expression-猫眼课题宝

编码活性大鼠胆碱乙酰转移酶的 cDNA 的完整序列：研究胆碱能表型表达可塑性的工具

DOI：

10.1002/jnr.490230304

发表时间：

1989

期刊：

Journal of Neuroscience Research

影响因子：

4.2

通讯作者：

中科院分区：

医学3区

文献类型：

作者：

A. Brice;S. Berrard;B. Raynaud;S. Ansieau;T. Coppola;Michel Weber;Jacques J. B. Mallet

已分离出编码活性大鼠胆碱乙酰转移酶（ChoAcTase；乙酰辅酶A：胆碱 O-乙酰转移酶，EC 2.3.1.6）完整序列的 cDNA 克隆。对推导的氨基酸序列的分析表明，与猪和果蝇酶分别有 85% 和 31% 的同一性。为了进一步阐明神经递质相关表型可塑性的分子基础，将 ChoAcTase mRNA 的表达与酪氨酸羟化酶 [TH;酪氨酸 3-单加氧酶、L-酪氨酸、四氢蝶啶：氧氧化还原酶（3-羟基化），EC 1.14.16.2]，来自在以下条件下生长的颈上神经节的神经元：（1）正常培养基，（2）高 K+ 培养基，和（3）补充有 50% 肌肉条件培养基（CM）的正常培养基。 TH mRNA 在所有三种培养基中均表达；在高 K+ 条件下，其水平上升，在 CM 存在下，其水平显着下降。 ChoAcTase mRNA 在 CM 中可见，但在正常和高 K+ 培养基中降至不可检测的水平。这些结果表明翻译或翻译后机制对于神经递质相关表型的调节并不起主要作用。

A cDNA clone encoding the complete sequence of an active, rat choline acetyltransferase (ChoAcTase; acetyl‐CoA:choline O‐acetyltransferase, EC 2.3.1.6) has been isolated. Analysis of the deduced amino acid sequence reveals 85% and 31% identity with the porcine and Drosophila melanogaster enzymes, respectively. To further elucidate the molecular basis of neurotransmitter‐related phenotypic plasticity, the expression of ChoAcTase mRNA was compared with that of tyrosine hydroxylase [TH; tyrosine 3‐monooxygenase, L‐tyrosine, tetrahydropteridine: oxygen oxidoreductase (3‐hydroxylating), EC 1.14.16.2], in neurons from superior cervical ganglia grown in the following conditions: (1) normal medium, (2) high K+ medium, and (3) normal medium supplemented with 50% muscle‐conditioned medium (CM). TH mRNA was expressed in all three media; its level rose in high K+ and decreased strikingly in the presence of CM. ChoAcTase mRNA could be visualized in CM, but fell to undetectable levels in normal and high K+ media. These results suggest that translational or post‐translational mechanisms do not play a major role for the modulation of neurotransmitter‐associated phenotype.

A chromosome 11-linked determinant controls fetal globin expression and the fetal-to-adult globin switch.