Cytosolic adenylyl cyclase defines a unique signaling molecule in mammals
Cytosolic adenylyl cyclase defines a unique signaling molecule in mammals
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DOI：
10.1073/pnas.96.1.79
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发表时间：
1999-01-05
影响因子：
11.1
通讯作者：
Levin, LR
中科院分区：
文献类型：
--
作者：
Buck, J;Sinclair, ML;Levin, LR
哺乳动物有9个差异调节的G蛋白反应跨膜腺苷酸环化酶亚型。我们现在描述存在一个独特的类哺乳动物腺苷酸环化酶，是可溶性的，G蛋白或毛喉素调节不敏感。北方分析表明可溶性腺苷酸环化酶（sAC）基因在睾丸中优先表达。从大鼠睾丸胞液中纯化的活性形式的sAC似乎是一个片段，来自全长蛋白，这表明sAC激活的蛋白水解机制。这两个推定的催化结构域的sAC是密切相关的蓝藻腺苷酸环化酶，细菌和哺乳动物的信号分子之间的进化联系。
Mammals have nine differentially regulated isoforms of G protein-responsive transmembrane-spanning adenylyl cyclases. We now describe the existence of a distinct class of mammalian adenylyl cyclase that is soluble and insensitive to G protein or Forskolin regulation. Northern analysis indicates the gene encoding soluble adenylyl cyclase (sAC) is preferentially expressed in testis. As purified from rat testis cytosol, the active form of sAC appears to be a fragment derived from the full-length protein, suggesting a proteolytic mechanism for sAC activation. The two presumptive catalytic domains of sAC are closely related to cyanobacterial adenylyl cyclases, providing an evolutionary link between bacterial and mammalian signaling molecules.