DIAGNOSIS OF MALARIA BY DETECTION OF PLASMODIUM-FALCIPARUM HRP-2 ANTIGEN WITH A RAPID DIPSTICK ANTIGEN-CAPTURE ASSAY
DIAGNOSIS OF MALARIA BY DETECTION OF PLASMODIUM-FALCIPARUM HRP-2 ANTIGEN WITH A RAPID DIPSTICK ANTIGEN-CAPTURE ASSAY
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DOI：
10.1016/s0140-6736(94)91520-2
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发表时间：
1994-03-05
期刊：
影响因子：
168.9
通讯作者：
HOFFMAN, SL
中科院分区：
文献类型：
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作者：
BEADLE, C;LONG, GW;HOFFMAN, SL
在肯尼亚进行了两项现场研究，在美国进行了一项实验性挑战研究，以评估基于外周血中恶性疟原虫富组氨酸蛋白2（PfHRP-2）定性检测的试纸抗原捕获测定法诊断恶性疟原虫感染的准确性。在这些研究中，该测定法对于检测大于60个恶性疟原虫无性寄生虫/μ L血液的灵敏度为96.5-100%，对于11-60个寄生虫/μ L血液的灵敏度为70-81%，对10个或更少寄生虫/μ L血液的敏感性为11-67%。特异性为95%（95%CI 85-105%; n = 20）之间的幼稚的美国志愿者，98%（96-101%; n=112）之间的志愿者暴露于恶性疟原虫感染的蚊子叮咬，和88%（84-92%; n=285）之间的肯尼亚人生活在一个地区的疟疾。我们的研究结果还表明，PfHRP-2抗原是不可检测的血液开始治疗性化疗后6天，并建议，这种循环抗原很少导致假阳性tests.The试纸条检测的灵敏度，特异性，简单，和速度可能使其成为一个重要的工具，在战斗中对疟疾。
Two field studies in Kenya and an experimental challenge study in the USA were done to assess the accuracy of a dipstick antigen-capture assay based on qualitative detection of Plasmodium falciparum histidine-rich protein 2 (PfHRP-2) in peripheral blood for diagnosis of P fafciparum infection.In these studies, the assay was 96.5-100% sensitive for detection of greater than 60 P falciparum asexual parasites/ mu L blood, 70-81% sensitive for 11-60 parasites/mu L blood, and 11-67% sensitive for 10 parasites or less/mu L blood. Specificity was 95% (95% CI 85-105%; n = 20) among naive American volunteers, 98% (96-101%; n=112) among volunteers exposed to the bite of P falciparium-infected mosquitoes, and 88% (84-92%; n=285) among Kenyans living in an area with holoendemic malaria. Our results also indicated that PfHRP-2 antigen was not detectable in blood 6 days after initiation of curative chemotherapy, and suggest that such circulating antigens rarely lead to false-positive tests.The dipstick assay's sensitivity, specificity, simplicity, and speed may make it an important tool in the battle against malaria.