Leukemia inhibitory factor and ciliary neurotropic factor promote the survival of sertoli cells and gonocytes in a coculture system
Leukemia inhibitory factor and ciliary neurotropic factor promote the survival of sertoli cells and gonocytes in a coculture system
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DOI：
10.1210/en.137.5.1885
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发表时间：
1996-05-01
期刊：
影响因子：
4.8
通讯作者：
VanDisselEmiliani, FMF
中科院分区：
文献类型：
--
作者：
DeMiguel, MP;DeBoerBrouwer, M;VanDisselEmiliani, FMF
白血病抑制因子（LIF）和睫状神经营养因子（CNTF）被发现是多效性调节支持细胞和生殖细胞的发展（均分离自新生大鼠睾丸）在共培养系统中，而IL-6，这个细胞因子家族的另一个成员，对这些细胞没有影响。LIF和CNTF以剂量和时间依赖性方式显著增强支持细胞的存活。LIF对Sertoli细胞的作用在培养3或6天后在1 ng/ml的浓度下是显著的，而CNTF在10 ng/ml时具有显著的作用。LIF和CNTF对支持细胞增殖均无影响。在添加LIF（7.5 ng/ml）或CNTF（10 ng/ml）的培养物中，增殖的生殖细胞（分离自5日龄大鼠睾丸）的存活率也显著较高。未发现这些细胞因子对增殖生殖细胞的有丝分裂活性有影响。然而，LIF（7.5 ng/ml）刺激静止的生殖细胞（分离自第1天的睾丸）的增殖后3天的文化。LIF（或CNTF）与成纤维细胞生长因子2（10 ng/ml）和钢因子（50 ng/ml）的组合没有进一步改善生殖细胞的长期培养。LIF和CNTF样蛋白的预期分子量（32，000和22，000道尔顿，分别在还原条件下）被发现在3日龄大鼠睾丸提取物的蛋白质印迹。总之，这些结果表明，LIF或CNTF可能在精子发生的开始发挥作用。生殖细胞和/或新生儿支持细胞上LIF或CNTF受体的表征将有助于更好地理解这些细胞因子在生殖系统中的生理作用。
Leukemia inhibitory factor (LIF) and ciliary neurotropic factor (CNTF) were found to be pleiotropic modulators of Sertoli cell and gonocyte development (both isolated from the neonatal rat testis) in a coculture system, whereas IL-6, another member of this cytokine family, had no effect on these cells. LIF and CNTF significantly enhanced the survival of the Sertoli cells in a dose- and time-dependent manner. The effect of LIF on the Sertoli cells was significant at a concentration of 1 ng/ml after 3 or 6 days of culture, whereas CNTF had a significant effect at 10 ng/ml. Neither LIF nor CNTF had an effect on Sertoli cell proliferation. The survival of proliferating gonocytes (isolated from 5-day-old rats testes) was also significantly higher in cultures to which LIF (7.5 ng/ml) or CNTF (10 ng/ml) was added. No effect of these cytokines was found on the mitotic activity of proliferating gonocytes. However, LIF (7.5 ng/ml) stimulated the proliferation of quiescent gonocytes (isolated from day 1 testes) after 3 days of culture. Combinations of LIF (or CNTF) with fibroblast growth factor 2 (10 ng/ml) and steel factor (50 ng/ml) did not further improve the long term culture of the gonocytes. LIF- and CNTF-like proteins of the expected molecular masses (32,000 and 22,000 daltons, respectively, under reducing conditions) were found by Western blotting in testicular extracts of 3-day-old rats. Taken together, these results indicate that LIF or CNTF may play a role at the start of the spermatogenesis. The characterization of receptors for LIF or CNTF on the gonocytes and/or neonatal Sertoli cells will aid in a better understanding of the physiological role of these cytokines in the reproductive system.