The RNA helicase DDX3 is required for ovarian development and oocyte maturation in Locusta migratoria
The RNA helicase DDX3 is required for ovarian development and oocyte maturation in Locusta migratoria
复制标题
RNA解旋酶DDX3是飞蝗卵巢发育和卵母细胞成熟所必需的
DOI：
10.1002/arch.21775
复制
发表时间：
2021
影响因子：
2.2
通讯作者：
Shuping Xing
中科院分区：
文献类型：
--
作者：
Junxiu Wang;Tingting Li;Sufang Deng;Enbo Ma;Jianzhen Zhang;Shuping Xing
DDX3是一个明确定义的DEAD - box RNA解旋酶亚家族，在RNA代谢、细胞周期、肿瘤发生、信号通路和生育等方面发挥多种功能。我们之前的研究表明，迁徙蝗中ddx3的同源基因lmddx3是普遍表达的，并且在睾丸和卵巢中丰度很高。flmddx3基因的敲低导致若虫的致死性表型，但其在生殖过程中的作用尚不清楚。因此，在本研究中，我们表征了lmddx3在雌性成虫中的表达，并分析了其在卵母细胞发育中的功能。lmddx3在所有组织中均有表达，在卵巢和后肠中表达量显著增加。在卵巢中，在成虫羽化后的第一天检测到强烈的表达水平，然后在卵母细胞发育过程中急剧下降。LmDDX3RNAi至少部分通过JH信号通路导致脂肪体卵黄蛋白原（Vg）表达降低，导致卵巢卵黄蛋白原受体（VgR）上调，从而阻碍卵巢发育和卵母细胞成熟。序列和系统发育分析表明，LmDDX3与白蚁DDX3亲缘关系较近。综上所述，这些数据揭示了lmddx3在调节vgr和vgr转录中的关键作用，vgr和vgr是卵黄形成的两个主要因子，是蝗虫卵巢发育和卵母细胞成熟的关键过程，并为蝗虫生物防治提供了新的假设靶点。
DDX3 represents a well‐defined subfamily of DEAD‐box RNA helicase and exerts multiple functions in RNA metabolism, cell cycle, tumorigenesis, signal pathway, and fertility. Our previous study has shown thatLmDDX3, the ortholog ofDDX3inLocusta migratoria, is ubiquitously expressed, and with a high abundance in testis and ovary. Knockdown ofLmDDX3results in a lethal phenotype in nymph, but it still remains unclear for its role in reproductive process. In this study, we therefore characterizedLmDDX3expression in female adult locust and analyzed its function in oocyte development.LmDDX3was expressed in all tissues examined with significant more transcripts in ovary and hindgut. In ovary, a strong expression level was detected at the day just after adult eclosion, and a dramatic reduction then occurred during the oocyte development.LmDDX3RNAi led to a reducedvitellogenin(Vg) expression in fat body via partially at least, the JH signaling pathway, and caused an upregulation ofvitellogenin receptor(VgR) in ovary, and thus blocked the ovarian development and oocyte maturation. Sequence and phylogenetic analysis indicated that LmDDX3 was closely related to termite DDX3. Taken together, these data reveal a critical role forLmDDX3in regulating the transcription ofVgandVgR, two major factors in vitellogenesis that is a key process required for ovary development and oocyte maturation in locust, and contribute thereof a new putative target for locust biological control.