A Kelch repeat protein, Cokel1p, associates with microtubules and is involved in appressorium development in Colletotrichum orbiculare.
A Kelch repeat protein, Cokel1p, associates with microtubules and is involved in appressorium development in Colletotrichum orbiculare.
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DOI：
10.1094/mpmi-23-1-0103
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
Ayumu Sakaguchi;T. Miyaji;G. Tsuji;Y. Kubo
中科院分区：
文献类型：
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作者：
Ayumu Sakaguchi;T. Miyaji;G. Tsuji;Y. Kubo
Kelch重复蛋白在多种生物体中是保守的，其中一些已知介导基本的细胞功能。我们从炭疽菌（Colletotrichum orbiculare）中分离到编码一种新的kelch重复蛋白的CoKEL1基因。对cokel1突变体的分析表明，cokel1参与附着胞发育和细胞壁合成。cokel1断裂突变体产生的附着胞形状不规则，胀气产生受损，突变体的附着胞很少能穿透宿主表皮细胞形成侵染菌丝。因此，与野生型相比，cokel1突变体对寄主叶片的致病性降低。此外，cokel1突变体对细胞壁降解酶更敏感，并且在细胞壁染色上显示出改变的标记。Cokel1p定位于营养菌丝的皮层微管和纺锤体微管。这些结果表明，Cokel1p是一种参与感染相关形态发生和致病性的微管相关蛋白。这是首次报道真菌植物病原体的致病性需要kelch重复蛋白。
Kelch repeat proteins are conserved in diverse organisms and some are known to mediate fundamental cellular functions. We isolated the gene CoKEL1, encoding a novel kelch repeat protein, from Colletotrichum orbiculare. Analysis of a cokel1 mutant indicated that CoKEL1 is involved in proper appressorium development and cell wall synthesis. Appressoria produced by cokel1 disruption mutants showed irregular shape and impairment of turgor generation and the mutant appressoria rarely penetrated to form infection hyphae in host epidermal cells. Accordingly, cokel1 mutants had reduced pathogenicity on host leaves compared with the wild type. Furthermore, the cokel1 mutant was more sensitive to cell-wall-degrading enzymes and showed altered labeling with the cell wall stain Calcofluor white. Cokel1p was localized on cortical and spindle microtubules in vegetative hyphae. These results suggest that Cokel1p is a microtubule-associated protein involved in infection-related morphogenesis and pathogenicity. This is the first report that a kelch repeat protein is required for the pathogenicity of a fungal plant pathogen.