Segmental expression of claudin proteins correlates with tight junction barrier properties in rat intestine
Segmental expression of claudin proteins correlates with tight junction barrier properties in rat intestine
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DOI：
10.1007/s00360-009-0440-7
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发表时间：
2010-04-01
影响因子：
2
通讯作者：
Amasheh, Salah
中科院分区：
文献类型：
--
作者：
Markov, Alexander G.;Veshnyakova, Anna;Amasheh, Salah
在管状上皮中，屏障功能以节段特异性方式变化。本研究的目的是将紧密连接蛋白的存在与大鼠肠道的细胞旁屏障特性关联起来。将十二指肠、空肠、回肠和结肠的组织段剥离粘膜下细胞层并安装在阻抗谱的尤斯室中以测量上皮电阻（R（epi））。同时，通过蛋白质印迹和免疫荧光共聚焦显微镜分析紧密连接蛋白的表达。结肠的 R (epi) 最高，其次是十二指肠、空肠和回肠。在小肠中，常见的跨上皮阻力（R (trans) 或 TER）高估了真实的 R (epi) 大约 60%。在结肠中，检测到“收紧”紧密蛋白 1、3、4、5 和 8 的最强表达。根据小肠最近端的 R (epi)，与空肠和回肠相比，十二指肠表现出“收紧”紧密蛋白的最高表达和介导通透性的紧密蛋白（即 Claudin-2、-7 和 -12）的最低表达。这些结果与十二指肠作为面对酸性胃内容物的第一部分的特定作用相对应。
In tubular epithelia, barrier function varies in a segment-specific way. The aim of this study was to correlate the presence of tight junction proteins and paracellular barrier properties along rat intestine. Tissue segments of duodenum, jejunum, ileum, and colon were stripped of submucosal cell layers and mounted in Ussing chambers for impedance spectroscopy to measure epithelial resistance (R (epi)). In parallel, expression of tight junction proteins was analysed by Western blots and immune fluorescence confocal microscopy. Colon showed highest R (epi), followed by duodenum, jejunum, and ileum. In small intestine, common transepithelial resistance (R (trans) or TER) overestimated true R (epi) by similar to 60%. In colon, strongest expression of "tightening" claudins 1, 3, 4, 5, and 8 was detected. In accordance with R (epi) the most proximal of the small intestinal segments, duodenum exhibited highest expression of "tightening" claudins and lowest expression of claudins mediating permeability, namely claudin-2, -7, and -12, compared to jejunum and ileum. These results correspond to the specific role of the duodenum as the first segment facing the acidic gastric content.