AL-PHA beads: bioplastic-based protease biosensors for global health applications
AL-PHA beads: bioplastic-based protease biosensors for global health applications
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AL-PHA 珠：用于全球健康应用的基于生物塑料的蛋白酶生物传感器
DOI：
10.1101/2020.06.18.159921
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Kelwick R
中科院分区：
文献类型：
--
作者：
Kelwick R
蛋白酶是多功能蛋白水解酶，在人类健康和疾病中具有复杂的作用。因此，蛋白酶生物传感器的开发可以有益于全球健康应用。为此，我们开发了先进的proteoLytic检测器PolyHydroxyAlkanoates（AL-PHA）珠-一个包含20多种低成本、可生物降解、基于生物塑料的蛋白酶生物传感器的库。广义上，这些生物传感器利用PhaC-报告融合蛋白，其结合到微生物制造的聚羟基链烷酸酯珠粒。在存在特异性蛋白酶的情况下，超折叠绿色荧光报告蛋白从AL-PHA珠上裂解-导致珠荧光损失。烟草蚀纹病毒（TEV）AL-PHA生物传感器检测至少1.85pM AcTEV的蛋白水解活性。AL-PHA珠也被设计用于检测曼氏血吸虫尾蚴转化液（SmCTF）样品中的尾蚴弹性蛋白酶，以及细胞外囊泡和细胞条件培养基样品中的癌症相关金属蛋白酶。我们设想AL-PHA珠可以进一步开发用于资源有限的环境。
Proteases are multi-functional proteolytic enzymes that have complex roles in human health and disease. Therefore, the development of protease biosensors can be beneficial to global health applications. To this end, we developed Advanced proteoLytic detector PolyHydroxyAlkanoates (AL-PHA) beads – a library of over 20 low-cost, biodegradable, bioplastic-based protease biosensors. Broadly, these biosensors utilise PhaC-reporter fusion proteins that are bound to microbially manufactured polyhydroxyalkanoate beads. In the presence of a specific protease, superfolder green fluorescent reporter proteins are cleaved from the AL-PHA beads – resulting in a loss of bead fluorescence. The Tobacco Etch Virus (TEV) AL-PHA biosensor detected the proteolytic activity of at least 1.85 pM of AcTEV. AL-PHA beads were also engineered to detect cercarial elastase fromSchistosoma mansoni-derived cercarial transformation fluid (SmCTF) samples, as well as cancer-associated metalloproteinases in extracellular vesicle and cell-conditioned media samples. We envision that AL-PHA beads could be further developed for use in resource-limited settings.
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影响因子：
4.8
作者：
Salter, JP;Lim, KC;McKerrow, JH
通讯作者：
McKerrow, JH
DOI：
10.1074/jbc.m202364200
发表时间：
2002-07
期刊：
The Journal of Biological Chemistry
影响因子：
--
作者：
Jason P. Salter;Youngchool Choe;H. Albrecht;C. Franklin;K. Lim;C. Craik;J. McKerrow
通讯作者：
Jason P. Salter;Youngchool Choe;H. Albrecht;C. Franklin;K. Lim;C. Craik;J. McKerrow
人胚胎肾细胞表达的基质金属蛋白酶的表征
影响因子：
2.7
作者：
Chi;Pel
通讯作者：
Pel
便当实验室
影响因子：
46.9
作者：
K. Aydin;Ç. Okuyaz;Kvlcm Gücüyener;A. Serdaroğlu;S. Akpek
通讯作者：
S. Akpek
影响因子：
81.5
作者：
McManus, Donald P.;Dunne, David W.;Zhou, Xiao-Nong
通讯作者：
Zhou, Xiao-Nong