Identification of a new member of transforming growth factor-beta superfamily in Drosophila:: The first invertebrate activin gene
Identification of a new member of transforming growth factor-beta superfamily in Drosophila:: The first invertebrate activin gene
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DOI：
10.1006/bbrc.1998.8678
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发表时间：
1998-05-29
影响因子：
3.1
通讯作者：
Wiggert, B
中科院分区：
文献类型：
--
作者：
Kutty, G;Kutty, RK;Wiggert, B
激活素是转化生长因子-β (TGP-β) 超家族的一个亚组，已在脊椎动物中对其在生长和发育中的作用进行了广泛研究。然而，激活素不被认为在无脊椎动物中表达。本文报道了第一个无脊椎动物激活素基因的鉴定。发现代表果蝇 4 号染色体 102 F 区域的基因组克隆编码假定的激活素 β。预测的蛋白质序列具有多碱性蛋白酶位点，该位点会生成包含 113 个氨基酸的成熟 C 端肽，与脊椎动物激活素 β(B)（抑制素 β(B)）序列的相似性超过 60%。 TGF-β 家族特征以及脊椎动物激活素中保守的所有 9 个半胱氨酸残基也存在于该成熟肽序列中。 Northern印迹和RT-PCR分析表明，激活素β基因在果蝇的胚胎、幼虫和成虫阶段都有表达。 (C) 1998 年学术出版社。
Activins, a subgroup of the transforming growth factor-beta (TGP-beta) superfamily, have been extensively studied in vertebrates for their roles in growth and development. However, activins are not thought to be expressed in invertebrates. The identification of the first invertebrate activin gene is reported here. A genomic clone representing 102 F region of the Drosophila chromosome 4 is found to encode a putative activin beta. The predicted protein sequence has a multibasic protease site that would generate a mature C-terminal peptide containing 113 amino acids showing >60% similarity to the vertebrate activin beta(B) (inhibin beta(B)) sequences. A TGF-beta family signature as well as all 9 cysteine residues conserved in the vertebrate activins are also present in this mature peptide sequence. Northern blot and RT-PCR analyses indicated that the activin beta gene is expressed in embryo, larva and adult stages of Drosophila. (C) 1998 Academic Press.