Regulating tension in three-dimensional culture environments.
Regulating tension in three-dimensional culture environments.
复制标题
调节三维文化环境中的紧张局势。
DOI：
10.1016/j.yexcr.2013.06.019
复制
发表时间：
2013
影响因子：
3.7
通讯作者：
Billiar,KristenLawrence
中科院分区：
文献类型：
--
作者：
Kural,MehmetHamdi;Billiar,KristenLawrence
几乎所有组织和器官的发育、修复和重塑过程都依赖于机械信号，包括外部载荷、细胞产生的张力和组织硬度。在过去的几十年里，人们对专门的二维培养系统中的机械转导途径有了很多了解;然而，人们也清楚地看到，细胞在二维和三维（3D）环境中的行为非常不同。三维体外模型将模拟体内基质环境的能力和细胞-基质相互作用的复杂性结合在一起。在这篇综述中，我们描述了张力在调节三维胶原蛋白和纤维蛋白基质中的细胞行为中的作用，重点是有效地利用全局边界条件来调节细胞群体共同作用所产生的张力。控制和测量这些3D培养系统中的张力的能力有可能增加我们对机械生物学的理解，并促进治疗患病组织的新方法的开发，并在再生医学和组织工程应用中指导细胞命运。
The processes of development, repair, and remodeling of virtually all tissues and organs, are dependent upon mechanical signals including external loading, cell-generated tension, and tissue stiffness. Over the past few decades, much has been learned about mechanotransduction pathways in specialized two-dimensional culture systems; however, it has also become clear that cells behave very differently in two- and three-dimensional (3D) environments. Three-dimensional in vitro models bring the ability to simulate the in vivo matrix environment and the complexity of cell–matrix interactions together. In this review, we describe the role of tension in regulating cell behavior in three-dimensional collagen and fibrin matrices with a focus on the effective use of global boundary conditions to modulate the tension generated by populations of cells acting in concert. The ability to control and measure the tension in these 3D culture systems has the potential to increase our understanding of mechanobiology and facilitate development of new ways to treat diseased tissues and to direct cell fate in regenerative medicine and tissue engineering applications.