A practical and fast isolation of 12 cyclolinopeptides (linusorbs) from flaxseed oil via preparative HPLC with phenyl-hexyl column
A practical and fast isolation of 12 cyclolinopeptides (linusorbs) from flaxseed oil via preparative HPLC with phenyl-hexyl column
复制标题
通过使用苯基己基柱的制备型 HPLC，实用、快速地从亚麻籽油中分离 12 种环亚麻肽 (linusorbs)
DOI：
10.1016/j.foodchem.2021.129318
复制
发表时间：
2021-02-26
期刊：
影响因子：
8.8
通讯作者：
Wang, Yong
中科院分区：
文献类型：
--
作者：
Liu, Xue;Cai, Zi-zhe;Wang, Yong
Linusorb，被称为环肽，是一组来自亚麻籽油的环状疏水肽，具有各种健康益处。然而，目前对linusorbs的生物活性和抗氧化能力的研究工作是有限的，因为它们的纯化和表征存在问题。建立了一种基于制备型HPLC同时分离12种Linusorbs的方法，并对溶剂选择、梯度洗脱程序、流速、上样量、上样浓度等因素进行了优化。最佳条件为：乙腈（ACN）与水的初始质量比为40%，乙腈质量比为80%，洗脱时间为21 min，流速为16 mL/min，上样量为12.5 mg，浓度为80 mg/mL（甲醇溶液）。使用离线MS/MS验证获得的12个linusorbs，记录纯度高于95.5%。该方法可以作为一种实用和快速的分离方法，使进一步调查的小linusorbs。
Linusorbs, known as cyclolinopeptides, are a group of cyclic hydrophobic peptides derived from flaxseed oil with various health benefits. However, the current research efforts on both the biological activities and antioxidant capacities of linusorbs are limited because of existing issues with their purification and characterization. A practical method based on preparative HPLC for isolating 12 linusorbs simultaneously was developed and factors such as the solvent selection, gradient elution program, flow rate, loaded mass, and loading concentration, were optimized. The optimum conditions were an initial acetonitrile (ACN) to water ratio of 40%, final ACN ratio of 80%, eluting time of 21 min, a flow rate of 16 mL/min, sample load of 12.5 mg, and concentration of 80 mg/mL (in methanol). The 12 linusorbs obtained were verified using off-line MS/MS, recording purities of above 95.5%. The method could serve as a practical and fast isolation method enabling further investigation of minor linusorbs.