REOXYGENATION INJURY IN ISOLATED HEPATOCYTES - CELL-DEATH PRECEDES CONVERSION OF XANTHINE DEHYDROGENASE TO XANTHINE-OXIDASE
REOXYGENATION INJURY IN ISOLATED HEPATOCYTES - CELL-DEATH PRECEDES CONVERSION OF XANTHINE DEHYDROGENASE TO XANTHINE-OXIDASE
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DOI：
10.1016/s0006-291x(88)81080-5
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发表时间：
1988-08-30
影响因子：
3.1
通讯作者：
LITTAUER, A
中科院分区：
文献类型：
--
作者：
DEGROOT, H;LITTAUER, A
缺氧孵育3小时后，从喂食大鼠中分离的肝细胞的复氧导致细胞活力显著增强。没有证据表明黄嘌呤氧化酶产生的活性氧参与了再氧合损伤，黄嘌呤脱氢酶转化为黄嘌呤氧化酶发生在几乎所有肝细胞都失去活力的时候。此外，黄嘌呤脱氢酶首先从严重损伤的细胞中释放，然后转化为氧化酶形式。结果表明，在完整的器官参与的活性氧，黄嘌呤氧化酶产生的，在复氧损伤可能只会发生时，在复氧，缺氧细胞损伤的部分组织已经进展到这样的程度，有一个显着的转换黄嘌呤脱氢酶黄嘌呤氧化酶。
Reoxygenation of isolated hepatocytes from fed rats after 3 h of anaerobic incubation led to a significantly enhanced loss of cell viability. No evidence for the participation of reactive oxygen species generated by xanthine oxidase in this reoxygenation injury was found. Conversion of xanthine dehydrogenase to xanthine oxidase occurred at a time when almost all of the hepatocytes had lost their viability. Furthermore, xanthine dehydrogenase was first released from the severely injured cells and then converted to the oxidase form. The results suggest that in the intact organ participation of reactive oxygen species, generated by xanthine oxidase, in reoxygenation injury may only occur when, upon reoxygenation, hypoxic cell injury in part of the tissue has progressed to such an extent that there is a significant conversion of xanthine dehydrogenase to xanthine oxidase.