INFLUENCE OF SLA HAPLOTYPE ON OVULATION RATE AND LITTER SIZE IN MINIATURE PIGS
INFLUENCE OF SLA HAPLOTYPE ON OVULATION RATE AND LITTER SIZE IN MINIATURE PIGS
复制标题
DOI：
10.1530/jrf.0.0820595
复制
发表时间：
1988-03-01
期刊：
影响因子：
--
通讯作者：
FORD, SP
中科院分区：
文献类型：
--
作者：
CONLEY, AJ;JUNG, YC;FORD, SP
从交配后第 2 天到第 6 天（交配第一天 = 第 0 天），从针对特定 SLA（猪 MHC）单倍型（a、c 和 d）培育的 3 种小型猪的母猪中收集全身血液并对其进行手术。通过计数黄体来确定排卵率，并将胚胎从子宫中冲出。黄体酮、雌二醇-17.β。通过 RIA 对血浆和子宫冲洗液中的雌酮和雌酮进行定量 SLAd/d 女性的排卵率高于 SLAa/a 或 SLAc/c 女性（分别为 11.cntdot.50 .+-. 0.87 vs 9.cntdot.11 .+-. 0.68 和 8.17 .+-. 0.83；P < 0.01）。雌酮高于雌二醇-17.β。全身血浆中（56.5 .+-. 6.4 vs 33.0 .+-. 4.7 pg/ml，P < 0.01），而雌二醇-17.beta。子宫潮红程度高于雌酮（19.8 .+-. 1.4 vs 14.9 .+-. 1.5 pg/角，P < 0.10）。全身孕酮浓度与交配后天数相关（r = 0.93，P < 0.01）。单倍型对测量的任何激素浓度都没有影响。对 SLAa/a、SLAa/c、SLAa/d、SLAd/c 和 SLAd/d 雄性和雌性的 99 只交配的产仔数进行了分析。 -/d 和 d/d 母猪或 d/d 公猪的产仔数大于所有其他交配的产仔数 (P < 0.05)。尽管 SLAd/d 母猪的排卵率较高，但父本 SLA 基因型对窝产仔数的显着影响表明 d 单倍型对胚胎存活的额外影响。
Systemic blood was collected from and surgery performed on sows of 3 strains of miniature swine bred for specific SLA (swine MHC) haplotypes (a, c and d) from Day 2 to Day 6 after mating (first day of mating = Day 0). Ovulation rate was determined by counting corpora lutea and embryos were flushed from the uterus. Progesterone, oestradiol-17.beta. and oestrone were quantitated in blood plasma and uterine flushings by RIA SLAd/d females had a higher ovulation rate than SLAa/a or SLAc/c females (11.cntdot.50 .+-. 0.87 vs 9.cntdot.11 .+-. 0.68 and 8.17 .+-. 0.83, respectively; P < 0.01). Oestrone was higher than oestradiol-17.beta. in systemic plasma (56.5 .+-. 6.4 vs 33.0 .+-. 4.7 pg/ml, P < 0.01) while oestradiol-17.beta. was higher than oestrone in uterine flushings (19.8 .+-. 1.4 vs 14.9 .+-. 1.5 pg/horn, P < 0.10). Systemic progesterone concentration was correlated with day after mating (r = 0.93, P < 0.01). There was no effect on haplotype on any of the hormone concentrations measured. Litter size was analysed from 99 matings amgonst SLAa/a, SLAa/c, SLAa/d, SLAd/c and SLAd/d sires and dams. Litter size from -/d and d/d sows or from d/d boars were larger (P < 0.05) than for all other matings. Although ovulation rate was higher in SLAd/d sows, the significant effect of sire SLA genotype on litter size suggests an additional effect of the d haplotype on embryonic survival.