Dissecting the protective effect of the seminal plasma spermadhesin PSP-I/PSP-II on boar sperm functionality
Dissecting the protective effect of the seminal plasma spermadhesin PSP-I/PSP-II on boar sperm functionality
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DOI：
10.2164/jandrol.05170
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发表时间：
2006-05-01
影响因子：
--
通讯作者：
Martinez, EA
中科院分区：
文献类型：
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作者：
Garcia, EM;Vázquez, JM;Martinez, EA
为了剖析PSP-Ⅰ/PSP-Ⅱ的保护活性，研究了分离的亚基PSP-Ⅰ和PSP-Ⅱ及其亲和纯化的胰蛋白酶肽和聚糖组分对高度稀释的猪精子的活力、线粒体活性和运动性的影响。高稀释度对对照精子产生负面影响。孵育的精子与PSP-Ⅰ/PSP-Ⅱ或其PSP-Ⅱ亚基有保护作用的精子功能，高线粒体膜电位，和精子活力。当精子与PSP-1亚基孵育时，这些效应不太明显。值得注意的是，精子与分离的PSP-I孵育可消除精子的活力。胰蛋白酶降解的PSP-I/PSP-II、PSP-I和PSP-II再现了天然蛋白质的作用。与用天然异源二聚体、胰蛋白酶消化的PSP-I/PSP-II、聚糖部分或不添加蛋白质孵育相同时间的精子相比，用聚糖耗尽的胰蛋白酶肽部分孵育5小时的精子保存了更高百分比的活精子。然而，精子活力随着添加的肽组分的浓度增加而降低。另一方面，与聚糖级分孵育的精子显示出比与肽级分孵育的精子更低的值。我们的结论是，PSP-Ⅰ/PSP-Ⅱ异二聚体精子黏附素的亚基发挥不同的活动对精子的功能。天然PSP-1/PSP-Ⅱ对高度稀释的猪精子的功能性的有益作用在很大程度上保留在其分离的PSP-Ⅱ亚基中，并且似乎不需要聚糖部分，这一发现表明肽部分可作为高度稀释的猪精子的潜在精子功能保留添加剂。
To dissect the protective activity of PSP-I/PSP-II, the effect of the isolated subunits PSP-I and PSP-II and their affinity-purified tryptic peptide and glycan fractions on the viability, mitochondrial activity, and motility of highly diluted boar spermatozoa was investigated. High dilution exerted a negative effect on control spermatozoa. Incubation of spermatozoa with PSP-I/PSP-II or with its PSP-II subunit had a protective effect on sperm functionality, high mitochondrial membrane potential, and sperm motility. These effects were less pronounced when spermatozoa were incubated with the PSP-I subunit. It was noteworthy that motility was abolished by incubation of spermatozoa with isolated PSP-I. Trypsin-degraded PSP-I/PSP-II, PSP-I, and PSP-II reproduced the effects of the native proteins. Incubating spermatozoa with the glycan-depleted trypticpeptide fraction of PSP-I/PSP-II for 5 hours preserved a higher percentage of viable spermatozoa than when sperm was incubated for the same time with the native heterodimer, trypsin-digested PSP-I/PSP-II, the glycan fraction or without added proteins. However, sperm motility decreased as the concentration of added peptide fraction increased. On the other hand, spermatozoa incubated with the glycan fraction showed lower values than spermatozoa incubated with the peptide fraction. We concluded that the subunits of the PSP-I/PSP-II heterodimeric spermadhesin exert different activities on sperm functions. The finding that the beneficial effect of the native PSP-I/PSP-II on the functionality of highly diluted boar spermatozoa is largely preserved in its isolated PSP-II subunit and does not appear to require the glycan moiety points to a peptide moiety as a potential sperm function-preserving additive of highly diluted boar spermatozoa.