ADHESION TO HELA-CELLS OF CAMPYLOBACTER-JEJUNI AND CAMPYLOBACTER-COLI OUTER-MEMBRANE COMPONENTS
ADHESION TO HELA-CELLS OF CAMPYLOBACTER-JEJUNI AND CAMPYLOBACTER-COLI OUTER-MEMBRANE COMPONENTS
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DOI：
10.1016/0923-2508(89)90014-4
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发表时间：
1989-01-01
影响因子：
2.6
通讯作者：
VERON, M
中科院分区：
文献类型：
--
作者：
FAUCHERE, JL;KERVELLA, M;VERON, M
空肠弯曲菌与C.大肠杆菌转化为上皮细胞被认为是肠炎的决定性步骤。在这项工作中，我们试图确定哪些细菌成分负责这种现象。外膜（OM）提取物从C. jejuni（3株）和C. coli（2株）。这些菌株已从腹泻发热患者的粪便中分离出来，并且能够粘附培养物中的HeLa细胞。将细菌OM提取物与培养物中的HeLa细胞孵育后，回收细菌粘附材料，进行电泳和免疫印迹。用兔抗全细菌细胞的抗血清显示细菌粘附抗原。发现范围为26至30 kDa的抗原级分优先结合HeLa细胞（细胞结合级分; CBF）。这些抗原是蛋白质，与鞭毛蛋白和脂多糖不同。细菌孵育兔抗血清提出了对同源CBF，不能结合HeLa细胞。此外，当抗血清被稀释时，抑制作用降低。在相同的条件下，兔抗血清提出了对92 kDa的非粘附OM部分没有阻止细菌结合HeLa细胞。
Adhesion of Campylobacter jejuni and C. coli to epithelial cells is thought to be a decisive step in enteritis. In this work, we tried to determine which bacterial components are responsible for this phenomenon. Outer membrane (OM) extracts were prepared from strains of C. jejuni (3 strains) and C. coli (2 strains). These strains had been isolated from stools of febrile patients with diarrhoea and were able to adhere to HeLa cells in culture. After incubation of bacterial OM extracts with HeLa cells in culture, bacterial adherent material was recovered, subjected to electrophoresis and immunoblotted. Bacterial adherent antigens were revealed by a rabbit antiserum raised against whole bacterial cells. Antigenic fractions, ranging from 26 to 30 kDa, were found to preferentially bind to HeLa cells (cell-binding fractions; CBF). These antigens were proteins and were distinct from flagellin and lipopolysaccharide. Bacteria incubated with a rabbit antiserum raised against homologous CBF, were unable to bind to HeLa cells. Moreover, the inhibitory effect decreased when the antiserum was diluted. Under the same conditions, a rabbit antiserum raised against a non-adherent OM fraction of 92 kDa did not prevent bacteria from binding to HeLa cells.