Signal sequence region of mitochondrial precursor proteins binds to mitochondrial import receptor.
Signal sequence region of mitochondrial precursor proteins binds to mitochondrial import receptor.
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DOI：
10.1073/pnas.90.8.3358
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发表时间：
1993-04
影响因子：
11.1
通讯作者：
H. Murakami;G. Blobel;D. Pain
中科院分区：
文献类型：
--
作者：
H. Murakami;G. Blobel;D. Pain
酵母的一个完整的线粒体膜蛋白（p32）先前已被分子克隆和测序，并建议作为线粒体输入受体的功能。然而，该蛋白质也被提出作为磷酸盐转运蛋白[Guérin，B.，Bukusoglu，C.，Rakotomanana，F. & Wohlrab，H.（1990）J. Biol. Chem. 265，19736-19741; Phelps，A.，Schobert，C.T. & Wohlrab，H.（1991）Biochemistry 30，248-252]。在这里，我们已经纯化p32后，其基因在大肠杆菌中的表达，并测定其能力，结合到各种前蛋白含有信号序列的蛋白质易位到线粒体，叶绿体，或内质网。我们的数据表明，p32包含一个结合位点的信号序列区的线粒体前蛋白。这些数据是一致的，与以前的分配p32作为进口受体，并讨论了关于这种蛋白质的磷酸盐转运明显冲突的分配。
An integral mitochondrial membrane protein (p32) of yeast has previously been molecularly cloned and sequenced and suggested to function as a mitochondrial import receptor. However, this protein has also been proposed to function as phosphate translocator [Guérin, B., Bukusoglu, C., Rakotomanana, F. & Wohlrab, H. (1990) J. Biol. Chem. 265, 19736-19741; Phelps, A., Schobert, C.T. & Wohlrab, H. (1991) Biochemistry 30, 248-252]. Here we have purified p32 after expression of its gene in Escherichia coli and assayed its ability to bind to various preproteins containing signal sequences for protein translocation into mitochondria, chloroplasts, or the endoplasmic reticulum. Our data suggest that p32 contains a binding site specific for the signal sequence region of mitochondrial preproteins. These data are consistent with the previous assignment of p32 as an import receptor and are discussed with regard to the apparently conflicting assignment of this protein as phosphate translocator.