In NMR tube transcription for rapid screening of RNA conformation
In NMR tube transcription for rapid screening of RNA conformation
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在 NMR 管转录中快速筛选 RNA 构象
DOI：
10.1080/15257770.2014.964412
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
G.
中科院分区：
文献类型：
--
作者：
Okui;S. and Kawai;G.
提出了一种利用核磁共振技术快速筛选RNA结构的简单方法。在NMR管中转录靶RNA，并且在不纯化的情况下测量其光谱。所提出的方法，在NMR管转录或INTT，适用于三个RNA的NMR谱已通过使用常规纯化的样品进行测量。通过实时测量，可以观察到亚氨基质子信号的增加和NTP信号的减少。经证实，INTT光谱一般类似于通过常规方法获得的那些。INTT可用于RNA折叠的初步筛选。
A simple method for rapid structure screening for RNA by NMR is proposed. Target RNA is transcribed in a NMR tube and its spectra are measured without purification. The proposed method, in NMR tube transcription or INTT, was applied for three RNAs for which NMR spectra have been measured by using the conventionally purified samples. By the real-time measuring, increase of imino proton signals and decrease of NTP signals can be observed. It was confirmed that INTT spectra are in general similar to those obtained by the conventional method. INTT can be used for the first-step screening of RNA folding.