Increased number and frequency of group 3 innate lymphoid cells in nonlesional psoriatic skin
Increased number and frequency of group 3 innate lymphoid cells in nonlesional psoriatic skin
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DOI：
10.1111/bjd.12658
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发表时间：
2014-03-01
影响因子：
10.3
通讯作者：
Bonefeld, C. M.
中科院分区：
文献类型：
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作者：
Dyring-Andersen, B.;Geisler, C.;Bonefeld, C. M.
背景银屑病是一种常见的免疫介导的炎症性疾病，影响皮肤和关节。白细胞介素（IL）‐23/IL‐17A轴和IL‐22在银屑病的发病机制中起关键作用。IL - 23反应性先天淋巴样细胞（ILCs）具有产生IL - 17和/或IL - 22的高能力，最近已被发现并与炎症性肠病有关。ilc在人体皮肤中的发生和作用尚不清楚。目的了解健康对照者和银屑病或镍过敏患者皮肤中不同ILC亚群的流行情况。方法对接触性镍过敏患者的健康皮肤、非病变性银屑病皮肤和病变性银屑病皮肤以及镍和凡士林暴露皮肤进行皮肤活检，分离淋巴细胞。用流式细胞术对细胞进行染色和鉴定。定量聚合酶链反应检测细胞因子和配体mRNA表达。结果我们发现三组ilc的成员存在于人类皮肤中。值得注意的是，与健康皮肤和接触性镍过敏患者的皮肤相比，银屑病患者的非病变皮肤和病变皮肤中RORγt+CD56+ILC3s的数量和频率均升高。此外，皮肤ILCs表达高水平的自然杀伤受体NKG2D。NKG2D与应激诱导的配体结合，包括主要组织相容性复合体I类相关链A，我们发现在牛皮癣患者的病变皮肤中，其表达强烈上调。结论il - c在人皮肤中存在，提示ror γ - t+CD56+ILC3可能参与银屑病的发病机制。
BackgroundPsoriasis is a common immune‐mediated inflammatory disease that affects the skin and joints. The interleukin (IL)‐23/IL‐17A axis and IL‐22 play key roles in the pathogenesis of psoriasis. IL‐23‐responsive innate lymphoid cells (ILCs) with a high capacity to produce IL‐17 and/or IL‐22 have recently been identified and associated with inflammatory bowel diseases. The occurrence and role of ILCs in human skin are poorly understood.ObjectivesTo describe the prevalence of the different ILC subpopulations in skin from healthy controls and patients with psoriasis or allergy to nickel.MethodsSkin biopsies were taken from healthy skin, nonlesional and lesional psoriatic skin, and nickel‐ and petrolatum‐exposed skin from patients with contact allergy to nickel, and lymphocytes were isolated. The cells were stained and characterized by flow cytometry. Cytokine and ligand mRNA expression were measured by quantitative polymerase chain reaction.ResultsWe found that members of the three groups of ILCs were present in human skin. Remarkably, the number and frequency of RORγt+CD56+ILC3s, which are known to produce IL‐22, were elevated in both nonlesional and lesional skin from patients with psoriasis compared with healthy skin and skin from patients with contact allergy to nickel. Furthermore, skin ILCs expressed high levels of the natural killer receptor NKG2D. NKG2D binds to stress‐induced ligands, including major histocompatibility complex class I‐related chain A, which we found to be strongly upregulated in lesional skin from patients with psoriasis.ConclusionThese results show that ILCs are present in human skin and indicate that RORγt+CD56+ILC3 may be involved in the pathogenesis of psoriasis.