Decrease of Perivascular Adipose Tissue Browning Is Associated With Vascular Dysfunction in Spontaneous Hypertensive Rats During Aging.
Decrease of Perivascular Adipose Tissue Browning Is Associated With Vascular Dysfunction in Spontaneous Hypertensive Rats During Aging.
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血管周围脂肪组织褐变的减少与衰老过程中自发性高血压大鼠的血管功能障碍有关
DOI：
10.3389/fphys.2018.00400
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发表时间：
2018
影响因子：
4
通讯作者：
Gao PJ
中科院分区：
文献类型：
--
作者：
Kong LR;Zhou YP;Chen DR;Ruan CC;Gao PJ
功能性血管周围脂肪组织（PVAT）通过机械支持和内分泌或旁分泌途径维持血管生理功能。PVAT表现出棕色脂肪组织（BAT）样特征，并且PVAT的布朗宁水平取决于解剖位置和物种。然而，PVAT布朗宁是否参与自发性高血压大鼠（SHR）的血管张力调节尚不清楚。在本研究中，我们的目的是说明老化对PVAT布朗宁和随后的血管反应在SHR的影响。本研究采用组织学染色和western blot方法检测了8周龄和16周龄SHR和Wistar-Kyoto（WKY）大鼠胸壁PVAT（tPVAT）的特征。我们还检测了血管反应性分析，以确定tPVAT对衰老过程中血管反应的影响。结果表明，tPVAT具有与BAT相似的表型，包括较小的脂肪细胞大小和阳性解偶联蛋白-1（UCP 1）染色。有趣的是，与WKY大鼠相比，8周龄SHR的tPVAT表现出增加的BAT表型标记物表达，而SHR的tPVAT的布朗宁水平从8周龄到16周龄比年龄匹配的WKY大鼠有更显著的下降。tPVAT对8周龄WKY和SHR的血管舒张作用无显著差异，而对16周龄SHR的血管舒张作用明显低于WKY。相反，tPVAT在8或16周龄WKY和SHR大鼠中显示出相似的血管收缩作用。此外，我们确定了一个重要的血管扩张剂腺苷，调节脂肪细胞布朗宁，可能是一个潜在的PVAT衍生的松弛因子。在SHR的tPVAT中，腺苷从8到16周龄显著降低。总之，衰老与SHR大鼠tPVAT布朗宁和腺苷产生的减少有关。这可能导致衰老过程中tPVAT对SHR的血管舒张作用减弱。
Functional perivascular adipose tissue (PVAT) is necessary to maintain vascular physiology through both mechanical support and endocrine or paracrine ways. PVAT shows a brown adipose tissue (BAT)-like feature and the browning level of PVAT is dependent on the anatomic location and species. However, it is not clear whether PVAT browning is involved in the vascular tone regulation in spontaneously hypertensive rats (SHRs). In the present study, we aimed to illustrate the effect of aging on PVAT browning and subsequent vasomotor reaction in SHRs. Herein we utilized histological staining and western blot to detect the characteristics of thoracic PVAT (tPVAT) in 8-week-old and 16-week-old SHR and Wistar-Kyoto (WKY) rats. We also detected vascular reactivity analysis to determine the effect of tPVAT on vasomotor reaction during aging. The results showed that tPVAT had a similar phenotype to BAT, including smaller adipocyte size and positive uncoupling protein-1 (UCP1) staining. Interestingly, the tPVAT of 8-week-old SHR showed increased BAT phenotypic marker expression compared to WKY, whereas the browning level of tPVAT had a more dramatic decrease from 8 to 16 weeks of age in SHR than age-matched WKY rats. The vasodilation effect of tPVAT on aortas had no significant difference in 8-week-old WKY and SHR, whereas this effect is obviously decreased in 16-week-old SHR compared to WKY. In contrast, tPVAT showed a similar vasoconstriction effect in 8- or 16-week-old WKY and SHR rats. Moreover, we identified an important vasodilator adenosine, which regulates adipocyte browning and may be a potential PVAT-derived relaxing factor. Adenosine is dramatically decreased from 8 to 16 weeks of age in the tPVAT of SHR. In summary, aging is associated with a decrease of tPVAT browning and adenosine production in SHR rats. These may result in attenuated vasodilation effect of the tPVAT in SHR during aging.
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影响因子：
7.8
作者：
Fleenor BS;Eng JS;Sindler AL;Pham BT;Kloor JD;Seals DR
通讯作者：
Seals DR
影响因子：
20.1
作者：
Chatterjee TK;Stoll LL;Denning GM;Harrelson A;Blomkalns AL;Idelman G;Rothenberg FG;Neltner B;Romig-Martin SA;Dickson EW;Rudich S;Weintraub NL
通讯作者：
Weintraub NL
影响因子：
29
作者：
Harms MJ;Ishibashi J;Wang W;Lim HW;Goyama S;Sato T;Kurokawa M;Won KJ;Seale P
通讯作者：
Seale P
影响因子：
16.6
作者：
Berbée JF;Boon MR;Khedoe PP;Bartelt A;Schlein C;Worthmann A;Kooijman S;Hoeke G;Mol IM;John C;Jung C;Vazirpanah N;Brouwers LP;Gordts PL;Esko JD;Hiemstra PS;Havekes LM;Scheja L;Heeren J;Rensen PC
通讯作者：
Rensen PC
影响因子：
37.8
作者：
Chang L;Villacorta L;Li R;Hamblin M;Xu W;Dou C;Zhang J;Wu J;Zeng R;Chen YE
通讯作者：
Chen YE