Substrate-Photocaged Enzymatic Fluorogenic Probe Enabling Sequential Activation for Light-Controllable Monitoring of Intracellular Tyrosinase Activity
Substrate-Photocaged Enzymatic Fluorogenic Probe Enabling Sequential Activation for Light-Controllable Monitoring of Intracellular Tyrosinase Activity
复制标题
底物光笼酶荧光探针能够顺序激活以光控监测细胞内酪氨酸酶活性
DOI：
10.1021/acs.analchem.0c00746
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发表时间：
2020
影响因子：
7.4
通讯作者：
Ronghua Yang
中科院分区：
文献类型：
--
作者：
Sheng Yang;Jiaxing Jiang;Anxin Zhou;Yibo Zhou;Wenling Ye;Dong-Sheng Cao;Ronghua Yang
酪氨酸酶（Tyrosinase，TYR）是参与黑素生成的关键酶，其过表达与黑色素瘤的发生密切相关。为了精确监测细胞内TYR活性，远程控制分子成像工具是非常有意义的，但仍有待探索。在这项工作中，我们提出了第一个photocaged酪氨酸酶荧光探针通过笼与光不稳定基团的酶探针的基板。由于顺序光和酶激活功能，该探针表现出光可控的“打开”响应对TYR具有良好的选择性和高灵敏度（检测限：0.08 U/mL）。荧光成像结果验证了笼状探针具有以光控方式可视化细胞内内源性酪氨酸酶活性的能力，从而为研究TYR相关的生理功能和病理作用提供了有前途的分子成像工具。此外，我们的顺序激活策略具有很大的潜力，开发更多的光可控酶荧光探针的时空分辨率。
Tyrosinase (TYR) is a crucial enzyme involved in melanogenesis, and its overexpression is closely associated with melanoma. To precisely monitor intracellular TYR activity, remote control of a molecule imaging tool is highly meaningful but remains to be explored. In this work, we present the first photocaged tyrosinase fluorogenic probe by caging the substrate of the enzymatic probe with a photolabile group. Because of the sequential light and enzyme-activation feature, this probe exhibits photocontrollable “turn on” response toward TYR with good selectivity and high sensitivity (detection limit: 0.08 U/mL). Fluorescence imaging results validate that the caged probe possesses the capability of visualizing intracellular endogenous tyrosinase activity in a photocontrol fashion, thus offering a promising molecule imaging tool for investigating TYR-related physiological function and pathological role. Moreover, our sequential activation strategy has great potential for developing more photocontrollable enzymatic fluorogenic probes with spatiotemporal resolution.