Discriminative analysis of Eucalyptus camaldulensis grown from seeds of various origins based on lignin components measured by pyrolysis-gas chromatography
Discriminative analysis of Eucalyptus camaldulensis grown from seeds of various origins based on lignin components measured by pyrolysis-gas chromatography
复制标题
基于热解-气相色谱法测定的木质素成分对不同来源种子生长的赤桉进行判别分析
DOI：
10.1016/s0165-2370(00)00137-6
复制
发表时间：
2001
影响因子：
6
通讯作者：
T. Ona
中科院分区：
文献类型：
--
作者：
H. Yokoi;Takahito Nakase;Y. Ishida;H. Ohtani;S. Tsuge;T. Sonoda;T. Ona
采用立式微炉裂解-气相色谱（Py-GC）结合主成分分析（PCA）技术，对不同产地赤桉（Eucalyptus camaldulensis）的种子进行了鉴别分析。在450°C下获得的桉树样品的热解图上，沿着可再现地观察到源自木质素的异戊基（S）和愈创木基（G）单元的许多特征峰以及源自纤维素和半纤维素的那些特征峰。首先，尝试在从木质素特征的热解产物的相对峰强度计算的S/G比的基础上进行桉树之间的判别分析。然而，仅通过观察到的S/G比难以明确规定种子的来源。然后，主成分分析应用于区分桉树样品的基础上的摩尔产率的峰，特别是来自木质素观察Py-GC。结果，即使生长在同一块地里，也能成功地鉴别桉树的起源。
Pyrolysis-gas chromatography (Py-GC) using a vertical microfurnace pyrolyzer combined with the principal component analysis (PCA) was applied to the discriminative analysis of Eucalyptus camaldulensis trees grown at the same field from the seeds of different origins. On the pyrograms of the Eucalyptus samples obtained at 450°C, many characteristic peaks derived from syringyl (S) and guaiacyl (G) units of lignin were reproducibly observed along with those derived from cellulose and hemicellulose. First, the discriminative analysis among the Eucalyptus trees was attempted on the basis of the S/G ratio calculated from the relative peak intensities of the pyrolyzates characteristic to lignin. However, it was difficult to make unequivocal specification for the origins of the seeds by the observed S/G ratio alone. Then, the PCA was applied to discriminate Eucalyptus tree samples based on the molar yields of the peaks specifically derived from lignin observed by Py-GC. As a result, the origin of Eucalyptus trees was successfully discriminated even grown at the same field.