Microscopy with ultraviolet surface excitation for rapid slide-free histology
Microscopy with ultraviolet surface excitation for rapid slide-free histology
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DOI：
10.1038/s41551-017-0165-y
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发表时间：
2017-12-01
影响因子：
28.1
通讯作者：
Levenson, Richard
中科院分区：
文献类型：
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作者：
Fereidouni, Farzad;Harmany, Zachary T.;Levenson, Richard
组织的组织学检查对于肿瘤和许多其他疾病的诊断和治疗至关重要，并且是临床前和基础研究的基础技术。然而，常用的明视野显微镜需要事先准备安装在载玻片上的微米厚的组织切片，这一过程可能需要数小时或数天，不仅会增加成本，还会延迟获取关键信息。在这里，我们介绍了一种简单、非破坏性的免载玻片技术，该技术可在几分钟内提供高分辨率的诊断组织学图像，类似于从传统苏木精和伊红组织学中获得的图像。我们将这种方法命名为紫外表面激发显微镜（MUSE），它还可以生成形状和颜色对比度信息。 MUSE依靠类似于280 nm的紫外光来限制常规荧光染色剂对组织表面的激发，并且对下游分子检测（包括荧光原位杂交和RNA测序）没有显着影响。 MUSE 承诺在最先进和资源匮乏的环境中提高患者护理的速度和效率，并为研究中的快速组织学提供机会。
Histological examination of tissues is central to the diagnosis and management of neoplasms and many other diseases and is a foundational technique for preclinical and basic research. However, commonly used bright-field microscopy requires prior preparation of micrometre-thick tissue sections mounted on glass slides-a process that can require hours or days, contributes to cost and delays access to critical information. Here, we introduce a simple, non-destructive slide-free technique that, within minutes, provides high-resolution diagnostic histological images resembling those obtained from conventional haematoxylin and eosin histology. The approach, which we named microscopy with ultraviolet surface excitation (MUSE), can also generate shape and colour-contrast information. MUSE relies on similar to 280 nm ultraviolet light to restrict the excitation of conventional fluorescent stains to tissue surfaces and it has no significant effects on downstream molecular assays (including fluorescence in situ hybridization and RNA sequencing). MUSE promises to improve the speed and efficiency of patient care in both state-of-the-art and low-resource settings and to provide opportunities for rapid histology in research.