cDNA cloning of S100 calcium-binding proteins from bovine periodontal ligament and their expression in oral tissues
cDNA cloning of S100 calcium-binding proteins from bovine periodontal ligament and their expression in oral tissues
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DOI：
10.1177/00220345980770090501
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发表时间：
1998-09-01
影响因子：
7.6
通讯作者：
Ishikawa, I
中科院分区：
文献类型：
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作者：
Duarte, WR;Kasugai, S;Ishikawa, I
牙周膜（PDL）是对牙齿功能至关重要的独特组织。然而，很少有人知道的分子机制控制PDL功能。为了在分子水平上鉴定PDL细胞，我们构建了牛PDL组织cDNA文库;然后，我们集中于分离S100钙结合蛋白（CaBP），因为它们介导Ca 2+信号传导并控制重要的细胞过程，如分化和代谢。克隆了两个牛S100 CaBP（S100 A4和S100 A2）的cDNA。北方印迹分析显示，S100 A4在萌出牙牙周膜中的表达最高。S100 A4在萌出牙PDL中的表达较低，在牙龈中的表达微弱。S100 A4在牙髓组织中表达也很高，其次是牙胚的牙乳头; S100 A2在PDLE和牙龈中表达很高。有趣的是，只有PDLE表现出S100 A4和S100 A2的高表达。PDLE还表达了最高水平的β-肌动蛋白（S100 A4的靶细胞骨架蛋白）。可以想象，S100 A4在PDLE中的高表达是PDL成熟和/或对咀嚼产生的机械应力的响应的结果。由于S100 A4在牙周膜和牙龈组织中的表达存在显著差异，我们认为S100 A4可能是区分牙周膜和牙龈组织的一个有用的标志物。
The periodontal ligament (PDL) is a unique tissue that is crucial for tooth function. However, little is known of the molecular mechanisms controlling PDL function. To characterize PDL cells at the molecular level, we constructed a cDNA Library from bovine PDL tissue; We then focused on the isolation of S100 calcium-binding proteins (CaBPs), because they mediate Ca2+ signaling and control important cellular processes such as differentiation and metabolism We screened the PDL cDNA library with a mouse S100A4 cDNA, and cloned the bovine cDNAs of two S100 CaBPs (S100A4 and S100A2). In Northern blotting analysis, the highest expression of S100A4 was detected in PDL from erupted teeth (PDLE). PDL from teeth under eruption (PDLU) showed a lower expression of S100A4, and its expression in gingiva was faintly detectable. S100A4 expression was also high in the pulp tissue followed by the dental papilla of the tooth germ; S100A2 expression was high in PDLE and gingiva. Interestingly, only PDLE exhibited a high expression of both S100A4 and S100A2. PDLE also expressed the highest level of beta-actin, a-target cytoskeletal protein for S100A4. It is conceivable that the high expression of S100A4 in PDLE is a result of the maturation of the PDL and/or a response to mechanical stress generated by mastication. Since there was a marked difference of S100A4 expression between PDL and gingiva, we propose that S100A4 could be a useful marker for distinguishing cells from these two tissues.