Virulence-associated gene profiling of Streptococcus suis isolates by PCR
Virulence-associated gene profiling of Streptococcus suis isolates by PCR
复制标题
DOI：
10.1016/j.vetmic.2005.12.013
复制
发表时间：
2006-06-15
影响因子：
3.3
通讯作者：
Valentin-Weigand, Peter
中科院分区：
文献类型：
--
作者：
Silva, Luciana M. G.;Baums, Christoph G.;Valentin-Weigand, Peter
强毒株猪链球菌的定义是有争议的。鉴定欧洲强毒株的一种成功方法是区分荚膜血清型（或相应的cps类型），随后检测毒力相关因子，即细胞外因子（EF、epf）、溶菌酶释放蛋白（MRP、mrp）和溶血素suilysin（SLY、sly）。本研究提出了一种新的多重PCR（MP-PCR）和一种mrp变异体PCR用于鉴定和表征强毒沙门氏菌。猪种。这些新方法被用于确定疾病与特定的毒力相关基因谱的关联。MP-PCR可鉴定S.通过对猪链球菌看家基因gdh的检测，对4种cps型（1、2、7、9型）的区分，以及epf、mrp、sly和arcA（精氨酸脱亚胺酶）的检测，对猪链球菌进行了初步的鉴定。suis）。此外，这项研究描述了第一个PCR检测至少6个mrp变异体的分化。MRP的相应大小的变体的表达被示出为六个mrp变体中的四个，但是在所研究的特定菌株中对于两个较大的mrp变体是检测不到的。这项研究的结果表明，cps 7菌株与肺炎有关，mrp的变异在这些菌株中非常明显。侵袭性肺炎和携带者S.用PCR方法对猪链球菌分离株进行检测，可将cps 1、cps 2、cps 7和cps 9菌株分为24种不同的基因型。45%的入侵S.本研究中调查的猪的疾病仅由这些基因型中的两种引起，即CPS 2/ MRP+/EPF+/SLY+和CPS 9/MRP*/EPF-/SLY+。因此，这项研究首次证明了绝大多数被调查的侵袭性cps 9菌株的特定毒力相关基因的统一谱。(c)2005 Elsevier B. V.保留所有权利。
Definition of virulent Streptococcus suis strains is controversial. One successful approach for identification of virulent European strains is differentiation of capsular serotypes (or the corresponding cps types) and subsequent detection of virulence-associated factors, namely the extracellular factor (EF, epf), the muramidase-released protein (MRP, mrp) and the hemolysin suilysin (SLY, sly). In this work we present a novel multiplex PCR (MP-PCR) and an mrp variant PCR for identification and characterization of virulent S. suis strains. These new methods were used to identify association of disease with particular profiles of virulence-associated genes. The MP-PCR allowed identification of S. suis through detection of the housekeeping gene gdh, differentiation of four cps types (1, 2, 7 and 9), and detection of epf, mrp, sly and arcA (arginine deiminase from S. suis). Furthermore, this study describes the first PCR assay for differentiation of at least six mrp variants. Expression of the corresponding size variants of MRP was shown for four of the six mrp variants, but was undetectable for the two larger mrp variants in the particular strains investigated. The results of this study suggest that cps7 strains are associated with pneumonia and that variation of mrp is very pronounced among these strains. Gene profiles of invasive, pneumonia and carrier S. suis isolates by combination of PCR assays allowed differentiation of 24 different genotypes among cps1, 2, 7 and 9 strains. Forty-five percent of the invasive S. suis diseases investigated in this study were caused by only two of these genotypes, namely cps2/ mrp+/epf+/sly+ and cps9/mrp*/epf-/sly+. Thus, this study demonstrates for the first time a uniform profile of the particular virulence-associated genes for the vast majority of the investigated invasive cps9 strains. (c) 2005 Elsevier B.V. All rights reserved.