In vitro oocyte maturation for the treatment of infertility associated with polycystic ovarian syndrome: the French experience
In vitro oocyte maturation for the treatment of infertility associated with polycystic ovarian syndrome: the French experience
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DOI：
10.1093/humrep/deh603
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发表时间：
2005-02-01
影响因子：
6.1
通讯作者：
Frydman, N
中科院分区：
文献类型：
--
作者：
Le Du, A;Kadoch, IJ;Frydman, N
背景：体外卵母细胞成熟（IVM）允许在体外受精中使用未成熟卵母细胞。IVM不需要卵巢刺激，因此可以提供给有卵巢过度刺激综合征风险的患者。方法：对33例多囊卵巢综合征（PCOS）患者进行45个周期的IVM治疗。结果：共获得卵母细胞复合物509个；24 h成熟的卵母细胞276个（54.2%），48 h成熟的卵母细胞45个（8.8%），24 h和48 h成熟的卵母细胞正常受精率分别为69.5%和73.3%。在获得的214个胚胎中，103个被移植，30个被冷冻。移植40例（2.5个胚胎/次）。11名妇女的β - hcg测试呈阳性（26.2%的妊娠/穿刺，27.5%的妊娠/移植），9名妇女有临床妊娠（20.0%的妊娠/穿刺，22.5%的妊娠/移植）。五个婴儿已经出生，一个正在怀孕。出生时进行的临床检查结果正常。结论：我们的研究结果表明，IVM可以作为传统体外受精和卵巢钻孔治疗多囊卵巢综合征的替代方案。IVM在治疗这一疾病中的作用有待进一步阐明。
Background: In vitro oocyte maturation (IVM) permits the use of immature oocytes in IVF. IVM does not require ovarian stimulation and so can be offered to patients at risk of ovarian hyperstimulation syndrome. Methods: For this indication, we carried out 45 cycles of IVM in 33 women with polycystic ovarian syndrome (PCOS). Results: A total of 509 cumulus-oocyte complexes was obtained; 276 (54.2%) oocytes matured in 24 h and 45 (8.8%) in 48 h. The normal fertilization (2PN) rate of oocytes matured in 24 and 48 h was 69.5 and 73.3% respectively. Among the 214 embryos obtained, 103 were transferred and 30 were frozen. Forty transfers were performed (2.5 embryos/transfer). Eleven women had a positive beta-hCG test (26.2% of pregnancies/puncture, 27.5% of pregnancies/transfer) and nine women had a clinical pregnancy (20.0% of pregnancies/puncture, 22.5% of pregnancies/transfer). Five babies have been born and one pregnancy is ongoing. Results of the clinical examination carried out at birth were normal. Conclusions: Our results show that IVM may be offered as an alternative to conventional IVF and to ovarian drilling in women with PCOS. The role of IVM in the therapeutic armamentarium for this condition should be further clarified.