Interactions between coat and scaffolding proteins of phage P22 are altered in vitro by amino acid substitutions in coat protein that cause a cold-sensitive phenotype.
Interactions between coat and scaffolding proteins of phage P22 are altered in vitro by amino acid substitutions in coat protein that cause a cold-sensitive phenotype.
复制标题
噬菌体 P22 的外壳蛋白和支架蛋白之间的相互作用在体外通过外壳蛋白中的氨基酸取代而改变，从而导致冷敏感表型。
作者：
Teschke,CM;Fong,DG
Cold-sensitive mutations in phage P22 coat protein cause the accumulation of precursor capsids in cells growing at the nonpermissive temperature (16 °C). The assembly of coat proteins which carry the substitutions threonine at position 10 to isoluecine (T10I), arginine at position 101 to cysteine (R101C), or asparagine at position 414 to serine (N414S) which cause cold-sensitivity has been investigated. All three proteins were found to fold into a monomeric species. Coat proteins carrying the amino acid substitutions T10I and R101C were not able to interact with scaffolding protein appropriately to initiate assemblyin vitrowhile coat protein carrying the substitution N414S was able to assemble; however, capsids formed of this protein had an increased affinity for scaffolding protein. These amino acid substitutions define two regions in coat protein that are essential for the interaction of coat protein with scaffolding protein at different stages in capsid maturation.
