Kinetics for exchange of imino protons in the d(C-G-C-G-A-A-T-T-C-G-C-G) double helix and in two similar helices that contain a G . T base pair, d(C-G-T-G-A-A-T-T-C-G-C-G), and an extra adenine, d(C-G-C-A-G-A-A-T-T-C-G-C-G).
Kinetics for exchange of imino protons in the d(C-G-C-G-A-A-T-T-C-G-C-G) double helix and in two similar helices that contain a G . T base pair, d(C-G-T-G-A-A-T-T-C-G-C-G), and an extra adenine, d(C-G-C-A-G-A-A-T-T-C-G-C-G).
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d(C-G-C-G-A-A-T-T-C-G-C-G) 双螺旋和包含 G 的两个相似螺旋中亚氨基质子交换的动力学。
DOI：
10.1021/bi00268a038
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发表时间：
1982
期刊：
影响因子：
2.9
通讯作者：
TinocoJr,I
中科院分区：
文献类型：
--
作者：
Pardi,A;Morden,KM;Patel,DJ;TinocoJr,I
Arthur Pardi,'Kathleen M. Morden, Dinshaw J. Patel, § 和 Ignacio Tinoco, Jr.* 摘要：测量来自各个碱基对的亚氨基质子的弛豫寿命：(I) 完美螺旋，d (CGCGAATTCGCG)，(II) 用 GT 碱基对替换 GC 碱基对的螺旋，d (CGTGAAT-TCGCG)，以及 (III) 完美螺旋具有错配的额外腺嘌呤碱基的螺旋，d (CGCAGAATTCGCG)。通过对这些双链体的亚氨基质子进行饱和恢复质子核磁共振实验来测量寿命。测量的亚氨基质子寿命与较高温度下的化学交换寿命和较低温度下的自旋晶格弛豫时间相对应。这些双链体的寿命比较表明，II 中 GT 碱基对的不稳定效应仅影响最近邻碱基对的打开率。对于螺旋 III，额外的腺嘌呤影响了螺旋中所有碱基对的打开率，因此
Arthur Pardi,'Kathleen M. Morden, Dinshaw J. Patel, § and Ignacio Tinoco, Jr.* abstract: The relaxation lifetimes of iminoprotons from individual base pairs were measured in (I) a perfect helix, d (CGCGAATTCGCG),(II) this helix with a GC base pair replaced with a GT base pair, d (CGTGAAT-TCGCG), and (III) the perfect helixwith an extra adenine base in a mismatch, d (CGCAGAATTCGCG). The lifetimes were measured by saturation recovery proton nuclear magnetic resonance experiments performed on the imino protons of these duplexes. The measured lifetimes of the imino protons were shown to correspond to chemical exchange lifetimes at higher temperatures and spin-lattice relaxation times at lower temperatures. Comparison of the lifetimes in these duplexes showed that the destabilizing effect of the GT base pair in II affected the openingrate of only the nearest-neighbor base pairs. For helix III, the extra adenine affected the opening rates of all the base pairs in the helix and thus