Inhibitory effect of voluntary running wheel exercise on the growth of human pancreatic Panc-1 and prostate PC-3 xenograft tumors in immunodeficient mice.
Inhibitory effect of voluntary running wheel exercise on the growth of human pancreatic Panc-1 and prostate PC-3 xenograft tumors in immunodeficient mice.
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DOI：
10.3892/or.19.6.1583
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发表时间：
2008-06
期刊：
影响因子：
4.2
通讯作者：
Xi Zheng;X. Cui;Mou-tuan Huang;Yue Liu;W. Shih;Y. Lin;Yaoping Lu;G. C. Wagner;A. Conney
中科院分区：
文献类型：
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作者：
Xi Zheng;X. Cui;Mou-tuan Huang;Yue Liu;W. Shih;Y. Lin;Yaoping Lu;G. C. Wagner;A. Conney
在本研究中，我们研究了自愿运动对免疫缺陷小鼠体内人类胰腺 Panc-1 和前列腺 PC-3 肿瘤形成和生长的影响。雌性严重联合免疫缺陷（SCID）小鼠皮下注射人胰腺癌Panc-1细胞，雄性SCID小鼠皮下注射人前列腺癌PC-3细胞。在向 SCID 小鼠皮下注射 Panc-1 或 PC-3 肿瘤细胞前一周开始，进行为期 63 天的自愿跑轮运动，抑制了 Panc-1 和 PC-3 肿瘤的生长。锻炼方案增加了雌性和雄性小鼠的食物和液体消耗。运动还减少了雌性小鼠的宫旁脂肪垫和雄性小鼠的睾丸脂肪垫的大小，但对体重没有影响。机制研究表明，自愿跑轮运动抑制增殖，表现为有丝分裂减少，运动方案还刺激细胞凋亡，表现为 Panc-1 和 PC-3 肿瘤中 caspase-3（活性形式）表达的增加。自愿跑轮运动使 Panc-1 和 PC-3 肿瘤中有丝分裂细胞/凋亡细胞的比例分别降低了 38% 和 32%。本研究证明了自愿运动对 SCID 小鼠异种移植模型中胰腺和前列腺肿瘤生长的抑制作用。
In the present study, we investigated the effect of voluntary exercise on the formation and growth of the human pancreas Panc-1 and prostate PC-3 tumors in immunodeficient mice. Female severe combined immunodeficient (SCID) mice were injected subcutaneously with human pancreatic cancer Panc-1 cells, and male SCID mice were injected subcutaneously with human prostate cancer PC-3 cells. Voluntary running wheel exercise for 63 days, starting one week before the subcutaneous injection of Panc-1 or PC-3 tumor cells into SCID mice, suppressed the growth of Panc-1 and PC-3 tumors. The exercise regimen increased the food and fluid consumption in the female and male mice. Exercise also decreased the size of the parametrial fat pads in the female mice and the paradidymis fat pads in the male mice, but there was no effect on the body weight. Mechanistic studies showed that voluntary running wheel exercise inhibited proliferation as reflected by a decreased mitosis, and the exercise regimen also stimulated apoptosis as reflected by the increased caspase-3 (active form) expression in the Panc-1 and PC-3 tumors. Voluntary running wheel exercise decreased the ratio of the percent mitotic cells/apoptotic cells in Panc-1 and PC-3 tumors by 38 and 32%, respectively. The present study demonstrated an inhibitory effect of voluntary exercise on the growth of pancreas and prostate tumors in a SCID mouse xenograft model.