Molecular functional analysis of auxin/indole-3-acetic acid proteins (Aux/IAAs) in plant disease resistance in cassava
Molecular functional analysis of auxin/indole-3-acetic acid proteins (Aux/IAAs) in plant disease resistance in cassava
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DOI：
10.1111/ppl.12970
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发表时间：
2020-01-01
影响因子：
6.4
通讯作者：
Shi, Haitao
中科院分区：
文献类型：
--
作者：
Fan, Shuhong;Chang, Yanli;Shi, Haitao
生长素/吲哚-3-乙酸蛋白(AUX/IAA)在生长素信号转导途径中发挥重要作用，广泛参与植物的发育和对非生物和生物胁迫的响应。木薯是热带地区最重要的生物质能源作物之一，但有关木薯中AUX/IAA蛋白的信息还很有限。本研究共鉴定了37个木薯Meaux/IAA基因家族成员，并对其进行了系统发育分析。Meaux/IAAs的转录水平通常受病原菌Xanthomonas axonopodis PV manihotis(XAM)的调控，其中一些特异地定位于细胞核。此外，Meaux/IAAs的过表达提高了烟草对Xam的抗性，而Meaux/IAAs沉默的植株在木薯中表现出对Xam的敏感性，这一点从叶片表型和叶片细菌种群中得到了证明。与抗病一致，MEAUX/IAA调控植物防御反应中病程相关基因(MePR)的转录水平、活性氧的积累和愈伤组织的发育。综上所述，基因图谱和功能分析确定了几个MEAUX/IAA是植物抗病的新成员，为进一步利用MEAUX/IAA提供了重要信息。
Auxin/indole-3-acetic acid proteins (Aux/IAAs) play important roles in auxin signaling pathways, with extensive involvement in plant development and plant response to abiotic and biotic stresses. Manihot esculenta (Cassava) is one of the most important biomass energy crops in tropical regions; however, the information about Aux/IAA proteins remain limited in cassava. In this study, 37 MeAux/IAA gene family members were identified in cassava and a phylogenetic analysis was performed. The transcript levels of MeAux/IAAs were commonly regulated by the pathogen Xanthomonas axonopodis pv manihotis (Xam), and some of them were specifically localized to the nucleus. Moreover, the overexpression of MeAux/IAAs confers an improved disease resistance against Xam in Nicotiana benthamiana, while MeAux/IAAs-silenced plants show disease sensitivity against Xam in cassava, as evidenced by the leaf phenotype and leaf bacterial population. Consistent with the disease resistance, MeAux/IAAs regulated the transcript levels of PATHOGENESIS-RELATED GENES (MePRs), reactive oxygen species accumulation and callose development in the plants' defense response. Taken together, gene profile and functional analysis identified several MeAux/IAAs as novel members in plant disease resistance, providing important information for further utilization of MeAux/IAAs.