Gene expression profiles of sodium-dependent vitamin C transporters in mice after alcohol consumption
Gene expression profiles of sodium-dependent vitamin C transporters in mice after alcohol consumption
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DOI：
10.1093/abbs/gmt099
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发表时间：
2013-11-01
影响因子：
3.7
通讯作者：
Duan, Xianglin
中科院分区：
文献类型：
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作者：
Guo, Xiaoqiang;Wang, Yuejia;Duan, Xianglin
酒精性肝病（ALD）是西方国家严重的肝脏疾病。我们之前的研究已经证明维生素C对ALD有保护作用。维生素C稳态受钠依赖性维生素C转运蛋白（SVCTs） 1和2的严格调控。但两种svct在ALD中的作用尚不清楚。在这项研究中，我们检测了小鼠饮酒后两种svct的表达模式。我们的研究结果表明，饮酒明显增加了肝脏中两种svct的表达，以及肾脏和肠道中SVCT1的表达，这对维生素C的吸收很重要。补充维生素C可提高血清维生素C含量，改善ALD症状。SVCT1介导的肠道吸收和肾脏再吸收是饮酒后血清维生素C含量升高的关键因素。我们提出活性氧和低维生素C浓度均可调节svct的表达，维生素C的保护作用是通过抑制缺氧诱导因子-1的稳定性来介导的。因此，我们的研究对于了解ALD中的维生素C稳态以及在ALD治疗中更好地使用其他抗氧化剂具有重要意义。
Alcoholic liver disease (ALD) is a serious liver problem in western countries. Our previous study has demonstrated that vitamin C plays a protective role in ALD. The vitamin C homeostasis is tightly regulated by sodium-dependent vitamin C transporters (SVCTs) 1 and 2. But the role of two SVCTs in ALD is less understood. In this study, we examined the expression patterns of two SVCTs in mice after alcohol consumption. Our results suggested that alcohol consumption obviously increased the expression of two SVCTs in liver and SVCT1 in kidney and intestine, which is important for vitamin C absorption. Vitamin C supplement increased the sera vitamin C content and ameliorated the symptom of ALD. Intestinal absorption and renal re-absorption mediated by SVCT1 are key factors to increase the sera vitamin C content after alcohol consumption. We proposed that both reactive oxygen species and low vitamin C concentration regulate the expression of SVCTs, and the protective role of vitamin C is mediated by suppressing the stability of hypoxia-inducible factor-1. Thus, our study is significant for the understanding of vitamin C homeostasis in ALD and for better use of other antioxidants in ALD therapy.